| 青蛤染色体制备及核型分析 |
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| 引用本文： | 段海宝,陈义华,董志国,张敏,葛红星,魏敏,周丽青,符翔超,孙泽鹏.青蛤染色体制备及核型分析[J].水产学报,2020,44(5):735-741. |
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| 作者姓名： | 段海宝 陈义华 董志国 张敏 葛红星 魏敏 周丽青 符翔超 孙泽鹏 |
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| 作者单位： | 江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港 222005;江苏海洋大学,江苏省海洋生物技术重点实验室,江苏连云港 222005;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室,上海 201306;江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港 222005;中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东青岛 266071;江苏海洋大学,江苏省海洋生物技术重点实验室,江苏连云港 222005 |
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| 基金项目： | 国家贝类产业技术体系专项(CARS-49)；江苏省高等学校自然科学研究重大项目(18KJA240001)；中国水产科学研究院黄海水产研究所基本科研业务费(20603022018004)；江苏省渔业科技类项目(Y2018-27)；江苏省优势学科海洋科学；江苏省研究生科研与实践创新计划项目(SJCX190979，SJCX190981) |
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| 摘 要： | 以青蛤成体鳃组织和性成熟的雌、雄青蛤性腺为材料,采用常规制片方法,分别制备青蛤二倍体染色体和单倍体染色体,统计染色体数目。结果显示,青蛤二倍体与单倍体染色体数目分别为2n=38和n=19;核型分析发现,青蛤核型组成为11m+6sm+2st,NF=76,未发现性染色体和随体染色体。本研究以青蛤成熟性腺为制备材料,成功制备单倍体染色体,与青蛤二倍体染色体互相印证,弥补了以往研究的不足,进一步丰富了对青蛤染色体数目及核型组成的认知。同时,也为青蛤染色体水平全基因组测序组装提供更加科学的基础资料。
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| 关 键 词： | 青蛤 染色体 性染色体 单倍体 核型分析 |
| 收稿时间： | 2019/10/16 0:00:00 |
| 修稿时间： | 2019/12/24 0:00:00 |
Chromosome preparation and karyotype analysis of Cyclina sinensis |
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| DUAN Haibao,CHEN Yihu,DONG Zhiguo,ZHANG Min,GE Hongxing,WEI Min,ZHOU Liqing,FU Xiangchao and SUN Zepeng.Chromosome preparation and karyotype analysis of Cyclina sinensis[J].Journal of Fisheries of China,2020,44(5):735-741. |
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| Authors: | DUAN Haibao CHEN Yihu DONG Zhiguo ZHANG Min GE Hongxing WEI Min ZHOU Liqing FU Xiangchao and SUN Zepeng |
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| Institution: | Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resurces, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China,Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China,Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China,Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China and Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China |
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| Abstract: | The clam Cyclina sinensis is an economically important shellfish in China, and it is urgent to explore the excellent germplasm resources of C. sinensis. This requires the support of accurate genetic and biological basic materials. In order to accurately understand the number of chromosome and its karyotype composition of C. sinensis, we prepared diploid chromosomes and haploid chromosomes from the gill tissue and mature gonad of C. sinensis, respectively. The results showed that the chromosome number of diploid was 2n = 38 and haploid was n = 19. On the basis of the above experiments, the well dispersed visual field of diploid chromosomes was measured by microphotography, and the karyotype was analyzed. The result of experiment indicates that the karyotype is 11m+6sm+2st, NF=76, and no heterosome and satellite chromosome were found. In this study, the haploid chromosome was successfully prepared by the mature gonads of the calm, which was to provide a new idea for molluscs chromosome studying. Moreover, the new discovery on chromosome number and karyotype of C. sinensis makes up for the results of previous studies, which was 2n = 36. And it contributed to the more correct direction of phylogenetic research and genetic research of bivalve molluscs, also provided more scientific basis for whole genome sequencing assembly of C. sinensis. |
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| Keywords: | Cyclaina sinensis chromosome heterosome haploid karyotype analysis |
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