| 大菱鲆脂质代谢相关基因PPARs的组织表达及其对高温胁迫的响应 |
| |
| 引用本文： | 赵亭亭,杨双双,马爱军,黄智慧,孙志宾,王新安,夏丹丹.大菱鲆脂质代谢相关基因PPARs的组织表达及其对高温胁迫的响应[J].水产学报,2020,44(4):515-522. |
| |
| 作者姓名： | 赵亭亭 杨双双 马爱军 黄智慧 孙志宾 王新安 夏丹丹 |
| |
| 作者单位： | 中国水产科学研究院黄海水产研究所,山东省海洋渔业生物技术与遗传育种重点实验室,青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛 266071;上海海洋大学水产与生命学院,上海 201306;青岛海洋科学与技术试点国家实验室,海洋生物学与生物技术功能实验室,山东青岛 266237;中国水产科学研究院黄海水产研究所,山东省海洋渔业生物技术与遗传育种重点实验室,青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛 266071;青岛海洋科学与技术试点国家实验室,海洋生物学与生物技术功能实验室,山东青岛 266237 |
| |
| 基金项目： | 现代农业产业技术体系专项(CARS-47-01)；青岛海洋科学与技术国家试点实验室“鳌山人才”培养计划项目(2017ASTCP-OS04)；国家自然科学基金(41706168)；山东省农业良种工程(2019LZGC013)；国家重点研发计划(2018YFD0900102)；烟台市科技计划(2018ZDCX021) |
| |
| 摘 要： | 为研究大菱鲆过氧化物酶体增殖物激活受体家族(PPARs)的组织分布和高温胁迫下PPARs在肾脏中的表达情况。实验采用荧光定量PCR(qPCR)技术检测PPARs基因3种亚型在大菱鲆不同组织中的表达情况以及高温胁迫下大菱鲆肾脏中PPARs的表达情况。qPCR结果显示,大菱鲆PPARs 3种亚型的组织分布存在显著差异。PPARα1和PPARα2在心脏中的表达量显著高于其他组织;PPARβ在大菱鲆的各个组织中普遍表达;PPARγ在大菱鲆的鳃中出现了显著性的高表达。大菱鲆肾脏中PPARs的mRNA水平随着温度升高呈现出不同的响应模式。PPARα随温度升高表达水平先显著降低,后有所升高;PPARβ的表达量在14、20、23和25℃时差异不显著,当温度升高至大菱鲆的致死温度28℃时,表达量出现了显著性的升高;PPARγ在14℃时表达水平很低,但随着温度的升高不断增加。研究表明,大菱鲆中存在PPARα、PPARβ和PPARγ3种亚型,而且三者可能以组织特异性的方式参与脂质代谢的调节,首次指出PPARs 3种亚型在温度胁迫中的表达变化,对PPARs的研究将推动鱼类脂代谢研究,揭示鱼类PPARs在脂质代谢调控以及响应逆境胁迫中的重要作用。
|
| 关 键 词： | 大菱鲆 脂质代谢 过氧化物酶体增殖物激活受体(PPARs) 基因表达 高温胁迫 |
| 收稿时间： | 2019/4/18 0:00:00 |
| 修稿时间： | 2019/6/5 0:00:00 |
Tissue expression of lipid metabolism related genes PPARs and their responses to heat stress in turbot (Scophthalmus maximus) |
| |
| ZHAO Tingting,YANG Shuangshuang,MA Aijun,HUANG Zhihui,SUN Zhibin,WANG Xin''an and XIA Dandan.Tissue expression of lipid metabolism related genes PPARs and their responses to heat stress in turbot (Scophthalmus maximus)[J].Journal of Fisheries of China,2020,44(4):515-522. |
| |
| Authors: | ZHAO Tingting YANG Shuangshuang MA Aijun HUANG Zhihui SUN Zhibin WANG Xin'an and XIA Dandan |
| |
| Institution: | Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China and Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory of Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China |
| |
| Abstract: | |
| |
| Keywords: | Scophthalmus maximus lipid metabolism peroxisome proliferator activated receptors (PPARs) gene expression heat stress |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
