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维生素C、E对中华绒螯蟹生殖性能的影响
引用本文:艾春香.维生素C、E对中华绒螯蟹生殖性能的影响[J].水产学报,2003,27(1):62-68.
作者姓名:艾春香
作者单位:1. 华东师范大学生命科学学院,上海,200062
2. NuGen Technologies,Inc.821 Industrial Road,San Carlos,CA 94070,U.S.A
基金项目:国家自然科学基金项目(30271012),上海市"曙光计划"(975G06)基金项目,高等学校博士点专项基金项目(2000026903),教育部回国留学人员科研启动基金项目,霍英东青年教师基金项目部分资助
摘    要:通过对河蟹雌性亲体投喂不添加或添加VC、VE的4组实验饲料,并以新鲜河蚌为对照,进行198d饲养试验,以雌蟹性腺系数、产卵力、孵化率以及各组织中SOD活性、MDA含量等指标作为判据,研究了VC、VE对雌蟹生殖性能的影响。结果表明,1(投喂添加0.5mg·(100g)-1VC饲料组)、2(投喂添加0.022mg·(100g)-1VE饲料组)和3(投喂添加0.5mg·(100g)-1VC饲料和0.022mg·(100g)-1VE饲料组)3个组雌蟹的产卵力分别为3620个卵细胞·g-1体重、3540个卵细胞·g-1体重、3710个卵细胞·g-1体重,孵化率分别是83.03%、84.06%、86.27%,均极显著高于4组(投喂未添加维生素饲料组)雌蟹的产卵力(2490个卵细胞·g-1体重)和孵化率(29.28%)(P<0.01),也显著高于5组(饲喂河蚌)雌蟹的产卵力(3010个卵细胞·g-1体重)和孵化率(71.12%)(P<0.05)。雌蟹卵巢中SOD活性,1、2和3组则比4组和5组显著低(P<0.05),分别为56.35Nu·mL-1、53.61Nu·mL-1和39.87Nu·mL-1,相应地,MDA含量分别为6.03nmol·mL-1、5.65nmol·mL-1和5.57nmol·mL-1;4组雌蟹,虽然其卵巢中SOD活性较高,达79.25Nu·mL-1,但因饲料中缺乏抗氧化性的VC、VE,引发卵子脂质过氧化,MDA含量显著增加(P<0.05),为23.18nmol·mL-1,导致卵质低下,从而影响了卵子孵化。综上所述,雌蟹饲料中添加适量的VC

关 键 词:中华绒螯蟹  维生素C、E  生殖营养需求  生殖性能
文章编号:1000-0615(2003)01-0062-07
收稿时间:2014/3/20 0:00:00
修稿时间:2002年1月16日

Effect of ascorbic acid and α-tocopherol in broodstock diet on reproductive performance of Eriocheir sinensis
AI Chun xiang.Effect of ascorbic acid and α-tocopherol in broodstock diet on reproductive performance of Eriocheir sinensis[J].Journal of Fisheries of China,2003,27(1):62-68.
Authors:AI Chun xiang
Institution:School of Life Science, East China Normal University, Shanghai200062, China
Abstract:Cultured trials lasting 198 days were conducted to determine the physiological effects of dietary vitamin C and E on reproductive performance of the Chinese mitten-handed crabs Eriocheir sinensis, with mean weight of 78. 21 ± 25. 68g, and female broodstocks were fed with four experimental diets of varying composition. The control group was fed with fresh clams. The gonadosomatic index ( GSI) , fecundity, hatchability of eggs, activities of SOD and the content of MDA in the different tissues, were used to evaluate the performance of the crabs fed w ith different diets. The results showed that the fecundity and hatchability of groups 1, 2 and 3 were 3 620 cell#g- 1( body weight) and 83. 03% ; 3 540 cell#g- 1 ( body weight ) and 84. 06% and 3 790 cell#g- 1 ( body weight) and 86. 27% respectively, which were very signif icantly higher than those of group 4 ( 2 490 cell# g - 1 body weight and 29. 28%) ( P < 0. 01) , and tho se of group 5 ( 3 010 cell#g - 1 body weight and 71. 12%) ( P < 0. 05) . The SOD activities in ovaries of groups 1, 2 and 3 were 56. 35Nu#mL- 1, 53. 61Nu#mL- 1 and 39. 87Nu#mL- 1 respectively, significantly lower than those of group 4 and 5 ( P < 0. 05) , and MDA contents were 6. 03nmol#mL - 1 , 5. 65nmol#mL - 1 and 5. 57nmol#mL - 1 respectively. Though the SOD activity in ovaries of group 4 was 79. 25Nu#mL- 1, peroxidation still took place because of def iciency in VC and VE, so MDA content in ovaries of group 4 was high, 23. 18nmol#mL- 1, resulting in very low hatchability of group 4. This experiment demonstrated that VC, VE supplemented in the diet s of female broodstocks can improve the reproductive performance.
Keywords:Eriocheir sinensis  vitamin E and C  broodstock nutrition  reproductive performance
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