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不同来源海洋弧菌微生物被膜对厚壳贻贝稚贝附着的影响
引用本文:黄道芬,梁箫,彭莉华,郭行磐,张德民,杨金龙.不同来源海洋弧菌微生物被膜对厚壳贻贝稚贝附着的影响[J].水产学报,2017,41(7):1140-1147.
作者姓名:黄道芬  梁箫  彭莉华  郭行磐  张德民  杨金龙
作者单位:1. 上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海 201306;上海海洋大学海洋生物科学国际联合研究中心,中国科学技术部,上海 201306;2. 浙江海洋高效健康养殖协同创新中心,浙江宁波,315211;3. 上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海 201306;上海海洋大学海洋生物科学国际联合研究中心,中国科学技术部,上海 201306;浙江海洋高效健康养殖协同创新中心,浙江宁波 315211
基金项目:国家自然科学基金(41476131);上海高校水产高峰学科建设项目;上海高校高原学科建设项目(海洋科学)
摘    要:为探讨广泛存在于近海环境中海洋弧菌和贝类附着的相互作用关系,实验从自然微生物被膜和厚壳贻贝成体肠道内分离了海洋弧菌,测定其种属及亲缘性,调查了这些不同来源弧菌形成的微生物被膜与厚壳贻贝稚贝附着的调控作用.结果显示,测试弧菌形成的微生物被膜密度随着初始细菌密度的增加呈现不同程度的增加.源于自然微生物被膜和贻贝肠道内的10株弧菌均能显著促进厚壳贻贝稚贝的附着,不同菌株形成微生物被膜的诱导活性存在显著差异,附着率变化范围为17%~67%,其中稚贝在Vibrio sp.17微生物被膜上的附着率为67%±2%.微生物被膜对稚贝附着诱导活性与被膜密度呈显著相关性,其中弧菌V.crassostreae ECSMB14106的相关性系数最高,为0.8992.此外,微生物被膜的诱导活性与弧菌的来源无关.本实验初步探明了海洋弧菌对厚壳贻贝稚贝附着的影响,有助于进一步理解微生物被膜调控厚壳贻贝的附着分子机理.

关 键 词:厚壳贻贝  稚贝  附着  海洋弧菌  微生物被膜
收稿时间:2017/2/8 0:00:00
修稿时间:2017/3/22 0:00:00

Effects of Vibrio biofilms of different sources on settlement of plantigrades of the mussel Mytilus coruscus
HUANG Daofen,LIANG Xiao,PENG Lihu,GUO Xingpan,ZHANG Demin and YANG Jinlong.Effects of Vibrio biofilms of different sources on settlement of plantigrades of the mussel Mytilus coruscus[J].Journal of Fisheries of China,2017,41(7):1140-1147.
Authors:HUANG Daofen  LIANG Xiao  PENG Lihu  GUO Xingpan  ZHANG Demin and YANG Jinlong
Institution:Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai 201306, China,Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China and Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai 201306, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China
Abstract:Marine biofilms play a crucial role in the settlement of plantigrades of the mussel Mytilus coruscus, but the effect of Vibrio derived from different sources on the settlement of plantigrades of the mussel M. coruscus remains unknown. Ten marine Vibrio species were isolated from natural biofilms and the intestine of the wild adult mussel M. coruscus and identified to investigate the relationship between these biofilms and mussel planitigrade settlement. The results showed that Vibrio biofilm cell densities increased with the increasing initial cell density. All test Vibrio biofilms can significantly promote mussel settlement, and the percentage of plantigrade settlement ranged from 17% to 67%. The inducing activities of these bacterial biofilms were significantly correlated to the microbial biofilm density, and the correlation coefficient in V. crassostreae ECSMB14106 was the highest (0.8992). In addition, the inducing activity of these bacterial strains was not correlated to the source of Vibrio. This study showed that marine Vibrio of the different sources induced mussel settlement and the finding is important for understanding the potential molecular mechanism of the biofilms mediating the settlement process in this species.
Keywords:Mytilus coruscus  plantigrade  settlement  marine Vibrio  biofilm
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