宽礁膜原生质体的分化与发育

为缩短传统育苗时间,开发室内种质保存技术,满足大规模栽培苗种需求,实验用4%的果胶酶和2%纤维素酶混合,将来自于不同月份和不同藻体部位的宽礁膜切段,分别在相同条件下解离成原生质体,详细研究了这些不同部位和不同生长时期细胞的再生、分化和发育途径。根据体细胞后代的外形、有无假根、有无公共膜、细胞大小和排列方式,以及它们最终的发育分化趋势,将宽礁膜原生质体发育方式分为8种结果,既可形成体细胞,也可形成生殖细胞。形成体细胞的发育途径又分3种类型7种结果,即细胞团、畸形苗和正常苗3种类型,其中发育成细胞团的类型又分为规则细胞团和不规则细胞团2种结果,发育成畸形苗的类型又分为假根为主的畸形苗、有类假根的畸形苗、无假根的畸形苗和管状畸形苗4种结果。各种发育类型与藻体的日龄、大小及部位有关,其原生质体直接发育形成正常形态藻体的发育方式只是8种发育结果的一种。

Differentiation and development of protoplasts of Monostroma latissimum

Monostroma latissimum is one edible Chlorophyta species,and is a potential new species for economic seaweed cultivation by fishermen in China,for viable protoplasts could be potentially used as a source for seed material of macrophytic marine algae cultivation and for other applied phycological research,so the aim of this study was to develop a new breeding method by culturing the protoplasts of M.latissimum,to form seedlings quickly,to shorten the traditional period of germlings cultivation,to maintain a s...