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硅烷化表面海洋细菌对厚壳贻贝稚贝附着的影响
引用本文:孙俊杰,张显,郭行磐,陈芋如,沈和定,丁德文,杨金龙.硅烷化表面海洋细菌对厚壳贻贝稚贝附着的影响[J].水产学报,2015,39(10):1530-1538.
作者姓名:孙俊杰  张显  郭行磐  陈芋如  沈和定  丁德文  杨金龙
作者单位:上海海洋大学,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,国家海洋局 第一海洋研究所,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室
基金项目:国家自然科学基金(41476131);上海市教委创新重点项目(14ZZ143);上海高校水产学一流学科建设项目
摘    要:为探讨厚壳贻贝稚贝对自然微生物膜中海洋细菌的附着行为反应,本论文研究了厚壳贻贝(Mytilus coruscus)稚贝附着与硅烷基化附着基表面、微生物膜密度以及细菌种属系统发育之间的相互关系。研究表明所测海洋细菌均能显著促进厚壳贻贝稚贝的附着;其中Staphylococcus sp. 1和Cobetia sp. 1表现出较低诱导活性,且这两株细菌形成的微生物膜密度与稚贝附着率之间无显著相关性;其他7株海洋细菌均表现出中等程度诱导活性,且所形成的微生物膜密度与稚贝附着率之间呈显著相关性。系统发育分析表明所测海洋细菌对厚壳贻贝稚贝附着的诱导活性与细菌种属无关。因而,硅烷基化表面的海洋附着细菌对厚壳贻贝附着有着显著性促进作用,本研究将为后续开展厚壳贻贝稚贝附着机制奠定了基础。

关 键 词:厚壳贻贝  稚贝  附着  硅烷基化  海洋细菌
收稿时间:2015/4/30 0:00:00
修稿时间:2015/8/16 0:00:00

Effects of marinebacteria from silanizing surfaces on plantigrade settlement of the mussel Mytilus coruscus
SUN Junjie,ZHANG Xian,GUO Xingpan,CHEN Yuru,SHEN Heding,DING Dewen and YANG Jinlong.Effects of marinebacteria from silanizing surfaces on plantigrade settlement of the mussel Mytilus coruscus[J].Journal of Fisheries of China,2015,39(10):1530-1538.
Authors:SUN Junjie  ZHANG Xian  GUO Xingpan  CHEN Yuru  SHEN Heding  DING Dewen and YANG Jinlong
Institution:Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Marine Ecology Research Center,The First Institute of Oceanography,State Oceanic Administration,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education
Abstract:To understand plantigrade settlement of the mussel Mytilus coruscus in response to the different bacterial strains isolated from silanizing surfaces, the relationship between bacterial biofilms and plantigrade settlement was conducted. All test marine bacteria were isolated from the biofilms formed on the silanizing surfaces and identified by 16S rDNA gene sequences. In the laboratory, the effects of monospecific bacterial biofilms on settlement of M. coruscus plantigrades were also investigated. The phylogenetic tree derived from the elected 16S rDNA sequence was constructed and the genetic distance was calculated. The results showed that the density of monospecific bacterial biofilms increased with the initial incubation density and all test bacterial biofilms showed significantly inducing activity on settlement of M. coruscus plantigrades. Among the 9 bacterial species, the biofilms of Staphylococcus sp. 1 and Cobetia sp. 1 induced the lower percentage of plantigrade settlement of 47% and 43%, and there was no signification correlation between the settlement and biofilm density. The biofilms of other 7 bacteria showed moderate inducing activity, and significant correlation between cell densities of these 7 bacterial strains and inducing activity were observed. The inducing activity of individual bacterial isolates was not correlated with their phylogenetic relationship. Thus, marine bacteria derived from biofilms developed on silanizing surfaces can promote the settlement of plantigrades of the mussel M. coruscus. Those results will provide some useful information to understand the relationship between bacterial biofilms and the settlement mechanism of M. coruscus plantigrades.
Keywords:Mytilus coruscus  plantigrade  settlement  silanisation  marine bacteria
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