| 中华绒螯蟹与日本绒螯蟹线粒体DNA12SrRNA序列的比较 |
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| 引用本文： | 高天翔.中华绒螯蟹与日本绒螯蟹线粒体DNA12SrRNA序列的比较[J].水产学报,2000,24(5):412-416. |
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| 作者姓名： | 高天翔 |
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| 作者单位： | 青岛海洋大学海洋渔业系,山东青岛 266003 |
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| 基金项目： | 日本学术振兴未来学术研究事业资助项目(97L00902) |
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| 摘 要： | 参考果蝇和蚤状Ｚａｏ的线粒体ＤＮＡ １２Ｓ ｒＲＮＡ序列进行了中华绒螯蟹与日本绒螯蟹的线粒体ＤＮＡ１２ＳｒＲＮＡ基因片段的引物设计、ＰＣＲ扩增及序列测定。两种的碱基序列长度相同，为４５７ｂｐ，其Ａ、Ｔ、Ｇ、Ｃ含量相似，分别为１５９ｂｐ（３４．７９％）、１７７ｂｐ（３８．７３％）、５１ｂｐ（１１．１６％）、７０ｂｐ（１５．３２％）和１５９ｂｐ（３４．７９％）、１７８ｂｐ（３８．９５％）５０ｂｐ（１０．９４％）、７０ｂｐ（１５．３２％）。中华绒螯蟹与日本绒螯蟹间有５处碱基序列差异，在９８ｂｐ、１５１ｂｐ、３１７ｂｐ和４１７ｂｐ碱基处为碱基转移，在２９４ｂｐ碱基处为碱基颠换。
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| 关 键 词： | 中华绒螯蟹 日本绒螯蟹 线粒体DNA 12SrRNA 序列 |
| 文章编号： | 1000-0615(2000)05-0412-05 |
| 收稿时间： | 2014/4/16 0:00:00 |
| 修稿时间： | 1999年12月24 |
Sequence comparison of mitochondrial DNA 12S rRNA region between Eriocheir sinensis and Eriocheir japonica |
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| GAO Tian xiang.Sequence comparison of mitochondrial DNA 12S rRNA region between Eriocheir sinensis and Eriocheir japonica[J].Journal of Fisheries of China,2000,24(5):412-416. |
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| Authors: | GAO Tian xiang |
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| Institution: | GAO Tian xiang 1,ZHANG Xiu mei 1,WATANABE Seiichi 2 |
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| Abstract: | The primers of Eriocheir sinensis and Eriocheir japonica were designed by using the sequences of mitochondrial DNA 12S rRNA region of Drosophila yakuba and Daphnia pulex. 457 base pairs of encoded 12S rRNA region of mitochondrial DNA were amplified and sequenced.The length of sequence of two species was the same,the A,T,G,C contents were similar,which were 159bp(34.79%),177bp(38.73%),51bp(11.16%),70bp(15.32%) and 159bp(34.79%),178bp(38.95%),50bp(10.94%),70bp(15.32%) respectively. Between E. sinensis and E.japonica,there were 5 different sites, that were transition sites at 98bp,151bp,317bp,417bp,and transversion site at 294bp. |
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| Keywords: | Eriocheir sinensis Eriocheir japonica mitochondrial DNA 12S rRNA sequence |
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