鲤脑垂体匀浆液和人绒毛膜促性腺激_省略_性腺激素及性类固醇激素含量的影响

雄鳗注射５－６次、雌鳗注射９－１０次鲤脑体匀浆液（ＣＰＥ）＋人绒毛膜促性腺激素（ＨＣＧ）能分别诱导精巢和卵巢发育成熟。在雌雄鳗鲡，注射ＣＰＥ＋ＨＣＧ可显著增加端脑、间脑、中脑和下丘脑ｍＧｎＲＨ的含量，而对后脑和延髓ｍＧｎＲＨ的影响较小；注射ＣＰＥ＋ＨＣＧ增加雄鳗后脑和延髓ｃＧｎＲＨ－Ⅱ含量，对雌鳗脑区ｃＧｎＲＨ－Ⅱ则无显著影响。雌雄鳗鲡每次注射ＣＰＥ＋ＨＣＧ后１天，血清促性腺激素（ＧｔＨ）急剧上升

Eeffect of combinative injection of carp pituitary extraction and human chorionic gonadotropin on brain gonadotropin-releasing hormone and serum gonadotropin and sex steroids contents in Anguilla japonica

The testes and ovarian development and maturation were achieved by combinative injection of carp pituitary extraction( CPE) and human chorionic gonadotropin ( HCG) for 5- 6 times in male and 9- 10 times in female Japanese eel ( Anguilla japonica ) . The mGnRH contents in the telencephalon, disencephalon, mesencephalon and hypothalamus signif icant ly increased after injection of CPE + HCG in male and female Japanese eel, but contents in the metencephalon and myelencephalon did not change. Injection of CPE HCG also increased the cGnRH- ò contents in the metencephalon and myelencephalon in male Japanese eel, but had no effect in the female. The serum GtH levels in male and female Japanese eel increased dramatically 1 day after each injection of CPE HCG, then gradually decreased 10 days after. However the serum GtH levels in female Japanese eel remained significantly higher than the corresponding control in 10 days after 7 inject ions. The serum testosterone concentration increased after injection of CPE HCG and kept on a high level in female Japanese eel, but did not change during the time of 1- 4 injection in male Japanese eel. The serum 17B- estradiol contents were not dif ferent with the corresponding controls during 4- 6 injections in female Japanese eel, but increased after 7 injections.