蟹源致病性拟态弧菌的粘附及侵袭特性

病原菌对机体的致病作用是由其产生的各种毒力因子共同作用的结果,其中病原菌对组织细胞或粘膜的表面粘附是造成机体感染的首要条件。病原菌的粘附因子主要包括菌毛粘附素和其它非菌毛粘附素(如鞭毛、外膜蛋白和血凝素等),其中以菌毛介导的病原菌对组织细胞的粘附是细菌在体内定居、增殖释放毒力因子发挥致病作用的关键。为了揭示拟态弧菌HX 4的致病机理,以经典粘附模式细胞HEp 2细胞作为体外细胞模型,探讨蟹源致病性拟态弧菌HX 4株的粘附特性以及受体物质和抗全菌抗体对细菌粘附作用的影响,同时采用庆大霉素清洗裂解培养法测定该菌对HEp 2细胞的侵袭力。结果显示,HX 4菌株能粘附HEp 2细胞,对HEp 2细胞具有侵袭力。细菌对HEp 2细胞的粘附方式为集聚性粘附,最佳粘附时间为1h,甘露糖和抗体能显著抑制该菌的粘附,表明HEp 2细胞上含有甘露糖样受体。该菌的粘附素除了主要是菌毛外,可能还含有鞭毛、外膜蛋白、血凝素等多种成分。

Adhesion and invasion of pathogenic Vibrio mimicus isolated from Eriocheir sinensis

It is well known that the most pathogenic bacteria can produce various virulence factors and they play function together and lead to the pathoenicity of bodis. The adhesion to cells or mucosa of pathogenic bacteria is the prime process which results in the infection of bodis.The adhesive factors of bacteria include pilus adhesion and non-pilus adhesion(such as flagella,outer membrane proteins and hemagglusion etc.).The adhesion to tissue-cells or mucosa of pathogenic bacteria mediating by pilus plays a key role in colonizing and propagating various virulence factors that result in the pathoenicity of bodis. In order to reveal the pathogensis mechanism of vibrio mimicus strain HX-4 in this study we used classic adhesive model-cell HEp-2 cells as cell model in vitro to test the adhesive ability of pathogentic vibrio mimicus HX-4 strain isolated from diseased Eriocheir sinensis to HEp-2 cells and the effect of receptors and antibody on the adhesion of the strain. At the same time, the invasive ability of the strain into HEp-2 cells was measured by lysiscounting assay. The results showed that vibrio mimicus HX-4 strain had invasive ability into HEp-2 cells, and it can adhere to HEp-2 cells in the way of assembly. That is to say vibrio mimicus HX-4 strain was positive bacteria of adhesion and the optimal adhesivetime was one hour. At the same time we also found that mannose and antibody could inhibit adherence effectively. These results demonstrated that there was D-mannose structure among the adhesive receptor structure and the adhesion of vibrio mimicus HX-4 strain may also include flagella, outer membrane proteins besides pilus.