聚甘露糖醛酸对中国对虾免疫相关酶活性和溶菌溶血活性的影响

在中国对虾的血清、肌肉和肝胰提取液中有酸性磷酸酶（ＡＣＰ）、碱性磷酸酶（ＡＬＰ）、过氧化物酶（ＰＯＤ），其中，ＡＣＰ和ＡＬＰ在肝脏中的活性最高。采用１．０％的聚甘露糖醛多糖作为免疫药物，对中国对虾进行注射刺激后，发现血清中的溶菌和溶血活性有显著提高；肝胰腺中的ＡＣＰ和ＡＬＰ活性也有明显增加，在７２ｈ，ＡＣＰ活性由对照组的３．２１Ｕ．１００ｍＬ＾－１提高到９．６９Ｕ．１００ｍｌ＾－１，ＡＬＰ活性由４

Effects of mannuronate polysaccharide on enzymes of Penaeus chinensis related with immune and hemolysis

Acid phosphatase (ACP), alkaline phosphatase (ALP) and peroxidase were found in the serum, meat and hepatopancreas extrace of Penaeus chinensis. The activities of ACP and ALP in the hepatopancreas were highest among the serum, meat and hepatopancreas. After Penaeus chinensis were stimulated by injection with 1. 0% polymannuronic acids, the activities of bacteriolysis and hemolysis in the serum increased, meat and the activities of ACP and ALP in the hepatopancreas enhanced obviously. ACP activity increased from 3. 21 (U. l00mL- 1 )of control group to 9.69(U. l00mL-1 ) and ALP activity increased from 4.09(U. l00mL- 1 ) to 13. 2(U' l00mL- 1 ) after 72 hours. However, the activities of these two enzymes in the serum and meat extract extract stable. The activities of POD in serum, and hepatopancreas changed little. So mannuronate polysaccharide could strengthen the immunological competence of Penaeus chinensis, and possessed some developmental value in aquatic culture.