草鱼γ样干扰素的体内诱导

采用细胞病变抑制等方法，在植物凝集素（Ｐhytoagglutinin,PHA)诱导的草鱼血清中检测到一种干扰素，理化和生物学性质鉴定表明，它不同于病秀导的α／β－干扰素，表现在对５６℃,pH２和０．１％ＳＤＳ敏感，其活性不能被α／β－干扰素抗体所中和，其诱生剂为非病毒性质的有丝分裂原，诱生作用受波酯（ＰＭＡ）和白细胞介素－２（ＩＬ－２）的调节，这些特性与人类和高等脊椎动物中报道的γ干扰素相符，表明是一种γ样性质的干扰素，草鱼γ样干扰素的体内诱导受环境温度，诱导时间，ＰＨＡ剂量等因素的影响，２５℃或３０℃水温诱导的干扰素活性明显高于１５℃或８℃，高剂量（每尾０.5-0.8mg)PHA诱导的干扰素活性明显太于低剂量（每尾０.1-0.3mg)，且达到活性高峰所需要的时间短，为３d，而低剂量为５d，ＰＭＡ和ＩＬ－２能促进草鱼γ样干扰素的诱生。

In vivo induction of interferon-γ like activity from Ctenopharyngodon idellus by phytoagglutinin

An interferon factor was detected in grass carp ( Ctenopharyngodon idellus) serum, induced in vivo with phytoagglutinin by cell pathological effect inhibition technique. Study on its biochemical and biological characteristics showed that it was different f rom the interferon-A/B induced by virus. It was sensitive to heat ( 56 e ) , pH2 and 0. 1% SDS, and its activity could not be neutralized by the antibody against interferon-A/B. It proved that the inducer was mitogen but w ith non-virus character, and the production could be modulated signif icant ly by the phorbol myristate ester and interleukin-2. All these were consistent with the interferon-Cin human, which indicated it was interferon-Clike activity. The induction was influenced by the temperature, inducing time and dosage of phytoagglutinin. The activity was higher at the temperature of 25 e or 30 e and at phytoagglutinin dosage of 0. 5- 0. 8 mg per f ish than that at 8 e or 15 e and at that of 0. 1- 0. 3 mg per fish. The inducing time when the activity was the highest was 3 days at high dosage of phytoagglutinin, which was shorter than that of 5 days at low dosage. The interferon induction could be promoted by the phorbo l myristate ester and interluekin-2.