| 厚壳贻贝5-羟色胺2A受体(5-HT2AR)基因克隆和时空表达 |
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| 引用本文： | 梁箫,陈珂,陈艳文,刘钰珠,李一峰,杨金龙.厚壳贻贝5-羟色胺2A受体(5-HT2AR)基因克隆和时空表达[J].水产学报,2018,42(12):1869-1879. |
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| 作者姓名： | 梁箫 陈珂 陈艳文 刘钰珠 李一峰 杨金龙 |
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| 作者单位： | 上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306,上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306,上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306;上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306 |
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| 基金项目： | 国家自然科学基金（41606147，31101885）；上海高校水产高峰学科建设项目 |
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| 摘 要： | 为探究5-羟色胺2A受体（5-HT 2A receptor， 5-HT2AR）基因在海水贝类生长发育中的作用，本研究通过RACE技术和实时荧光定量PCR（qRT-PCR）克隆了厚壳贻贝5-HT2AR 基因的cDNA全长，并分析该基因的时空表达。结果显示，5-HT2AR基因全长2 636 bp，开放阅读框（ORF）2 124 bp，共编码707个氨基酸。序列分析结果显示该序列与人、小鼠、斑马鱼、长牡蛎和虾夷扇贝等物种分别具有45%、45%、48%、49%和67%的同源性。厚壳贻贝雌雄成体各组织和器官中均有5-HT2AR基因表达，雄性中的鳃表达量最高，而在雌性鳃、外套膜和性腺中的表达稍高于其他组织和器官；推测该基因可能与厚壳贻贝的摄食、对外界环境的感知及促进卵母细胞成熟有关。5-HT2AR基因在厚壳贻贝各发育阶段均有表达，且稚贝的表达量为眼点幼虫的1.4倍，推测5-HT2AR基因可能参与了调控厚壳贻贝幼虫的生长发育过程。本研究为进一步了解5-HT基因家族在双壳贝类中的功能奠定了基础。
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| 关 键 词： | 厚壳贻贝 5-HT2AR基因 基因克隆 表达分析 |
| 收稿时间： | 2018/8/14 0:00:00 |
| 修稿时间： | 2018/10/8 0:00:00 |
5-HT2AR gene clone and temporal-spatial expression in the mussel Mytilus coruscus |
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| LIANG Xiao,CHEN Ke,CHEN Yanwen,LIU Yuzhu,LI Yifeng and YANG Jinlong.5-HT2AR gene clone and temporal-spatial expression in the mussel Mytilus coruscus[J].Journal of Fisheries of China,2018,42(12):1869-1879. |
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| Authors: | LIANG Xiao CHEN Ke CHEN Yanwen LIU Yuzhu LI Yifeng and YANG Jinlong |
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| Institution: | International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China and International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | To understand the role of serotonin 5-HT2A receptor (5-HT2AR) gene in the growth and development of the mussel Mytilus coruscus, we cloned the 5-HT2AR gene cDNA full length of M. coruscus through RACE technology and analyzed the temporal and spatial expression of this gene via the real-time fluorescent quantitative PCR (qRT-PCR). The full length of 5-HT2AR gene was 2 636 bp and 2 124 bp of open reading frame (ORF) encoding 707 amino acids. Homologous analysis of the amino acids of 5-HT2AR gene in M. coruscus shared high similarity with Homo sapiens (45%), Mus musculus (45%), Danio rerio (48%), Crassostrea gigas (49%) and Mizuhopecten yessoensis (67%), respectively. This study showed that 5-HT2AR mRNA expression was detected in all tissues and the highest expression was found in gill and female gonad, indicating that 5-HT2AR gene may play an important role in perception of the external environment and promotion of mature oocytes. The 5-HT2AR mRNA was expressed at all developmental stages, suggesting that 5-HT2AR gene may be involved in the regulation of the growth and development. Taken together, our findings may provide new insights into clarifying the function of 5-HT gene family in molluscs. |
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| Keywords: | Mytilus coruscus 5-HT2AR gene gene cloning expression analysis |
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