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中华鲟幼鱼鳃丝Na+,K+-ATPase α 亚基渗透调节的分子机制初步研究
引用本文:封苏娅,赵峰,庄平,章龙珍.中华鲟幼鱼鳃丝Na+,K+-ATPase α 亚基渗透调节的分子机制初步研究[J].水产学报,2012,36(9):1386-1391.
作者姓名:封苏娅  赵峰  庄平  章龙珍
作者单位:华东理工大学生物工程学院;中国水产科学研究院东海水产研究所,农业部东海与远洋渔业资源开发利用重点实验室;中国水产科学研究院东海水产研究所,农业部东海与远洋渔业资源开发利用重点实验室;中国水产科学研究院东海水产研究所,农业部东海与远洋渔业资源开发利用重点实验室
基金项目:国家自然科学基金项目(31101881);公益性行业(农业)科研专项(201203065);长江口中华鲟自然保护区专项
摘    要:为了研究盐度10条件下中华鲟幼鱼鳃丝Na+,K+-ATPase(NKA)α亚基的分子调节机制,采用克隆方法得到中华鲟幼鱼鳃丝NKAα亚基部分基因序列,在鲟鱼转移后3,6,12,24,48,72和96 h检测实验组(淡水-盐水)和对照组(淡水-淡水)中华鲟幼鱼鳃丝NKAα亚基的mRNA表达水平、鳃丝NKA活性、血清Na+、Cl-的浓度和渗透压。结果显示,在转移后0~12 h,实验组中的mRNA表达量与酶活性为适应盐度变化而显著增加(P<0.05),离子浓度和渗透压均显著上升(P<0.05);在转移后12~24 h,mRNA表达和酶活性开始降低到一个较低值,但仍然显著高于对照组的值(P<0.05),离子﹑渗透压的变化与酶活性及mRNA变化趋势一致,这表明中华鲟鳃丝NKA在应对盐度变化时通过改变mRNA表达量来增加酶活性,高活性NKA调节血清离子和渗透压平衡。

关 键 词:中华鲟  Na+  K+-ATPase  α亚基  盐度  基因克隆  表达
收稿时间:2011/10/17 0:00:00
修稿时间:4/6/2012 12:00:00 AM

Preliminary studies on molecular mechanism of salinity regulation of Na~+, K~+-ATPase α-subunit in gills of juvenile Chinese sturgeon(Acipenser sinensis)
FENG Su-y,ZHAO Feng,ZHUANG Ping and ZHANG Long-zhen.Preliminary studies on molecular mechanism of salinity regulation of Na~+, K~+-ATPase α-subunit in gills of juvenile Chinese sturgeon(Acipenser sinensis)[J].Journal of Fisheries of China,2012,36(9):1386-1391.
Authors:FENG Su-y  ZHAO Feng  ZHUANG Ping and ZHANG Long-zhen
Institution:1.School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China; 2.Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture, East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China)
Abstract:In order to explore the molecular regulation mechanism of Na+,K+-ATPase(NKA)α-subunit in gills of juvenile Chinese sturgeon at salinity 10,partial NKA α-subunit gene sequence was cloned,and the abundance of α-subunit mRNA,NKA activity,ion concentration and osmolality were examined at 3,6,12,24,48,72,96 hours post-transfer.Results showed that:in experimental group(fresh water-salinity water,FW-SW),the expression of NKA α-subunit mRNA and NKA activity increased significantly(P<0.05)during the adaptation phase(0-12 h),and the concentration of Na+,Cl-and osmolality rose to a maximum level at 12 h post-transfer.In regulatory phase(12-24 h),the amounts of NKA α-subunit mRNA and NKA activity fell to a relatively lower level which was still higher than that of fish in control group(P<0.05).The change both in ion concentration and osmolality were in accordance with the change of NKA and mRNA expression.In conclusion,gill Na+,K+-ATPase in Chinese sturgeon plays an important role in osmolality regulation by changing α-subunit mRNA expression.The different α-subunit mRNA expression can lead to the variation of enzyme activity which can further regulate the ion concentration and osmolality.
Keywords:Acipenser sinensis  Na+  K+-ATPase α-subunit  salinity  gene clone  expression
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