海带褐藻糖胶的研究

本工作研究了我国人工养殖的海带叶片中褐藻糖的分布，及海带综合利用工业的废料碱凝沉物中褐藻糖胶的存在量和分离条件。海带叶片中的褐藻糖含量分布边缘比中部多，并从基部向尖部增加，即由0．3％增至1％以上。试验了用十六烷氯化吡啶法(CPC法)和乙醇沉淀法从碱凝沉物中分离褐藻糖胶的条件。两种方法的褐藻糖胶获得率分别为5．6％和4．4％(对干碱凝沉物)，其中的褐藻糖含量分别为24．7％和28．7％。碱凝沉物中的褐藻糖含量为1．4～3．5％。还研究了从褐藻糖胶以酸水解制取褐藻糖的诸条件，并分离出纸层析纯的褐藻糖结晶。

A STUDY ON THE FUCOIDAN OF LAMINARIA JAPONICA

That paper deals with the studies on the distributiou of fucose in the frond of cul-tivated Laminaria japonica on the methods for isolating fucoidan from the alkali--coagu-lants--the waste residue from the comprehenesive utilization of Laminaria. The content of fucose in Laminaria frond is higher in the marginal portion than inthe middle portion, and longitudinally it increases from 0. 3% in the basal part to morethan 1% in the distal part. (Fig. 1 and Table 1) Two methods of isolating fucidan from the alkali-coagulants were tested, i. e., thecetyl pyrimidium chlorid (CPC) precipitation method and the alcohol arecipitationmethod. (Fig. 2, b) The yields of fucoidan obtained by the two methods are 5.6% and4.4% (to the alkalicoagulants at dry state), and the fucose content are 24.7% and28.7% re,spectively. (Table 4, 5, 6) The content of fucose in alkali--coagalants amounts to 1.4--3.5%. (Table, 3) On acid hydrolysis of the crude fucoidan and subscquent treatment including deioni-zation, evaporation, etc. were applied, the fucose crylals settled out in absolute alcoholafter several days with a trace amount of galactose detected by paper chromatograph andwere further purified by dissolving with methanol--acetone mixture and thus rearystal-lization occurred with only a single spot of fucose shown on paper chromatograpgh.