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剑尾鱼RW-H近交系的遗传结构分析
引用本文:李霞.剑尾鱼RW-H近交系的遗传结构分析[J].水产学报,2004,28(4):365-370.
作者姓名:李霞
作者单位:1. 中国水产科学研究院珠江水产研究所,广东,广州,510380;上海水产大学生命与科学技术学院,上海,200090
2. 中国水产科学研究院珠江水产研究所,广东,广州,510380
基金项目:国家公益研究专项资金(34043),广东省重点项目(2KB07701T)
摘    要:筛选31条随机引物对剑尾鱼的第8代近交RW-H系A、B、C3窝共11尾剑尾鱼个体基因组DNA进行RAPD扩增,计算近交系个体间及不同窝间的相似系数及遗传距离。结果筛选的31条引物共扩增出383条带,扩增片段的大小在0.2~3kb。其中多态性带30条,多态性带频率在0~58.33%,平均为7.83%。近交系剑尾鱼不同个体拥有相同条带的比率较高。计算得到近交系的平均相似系数(S)为0.9829(0.9716~0.9960),平均等位基因频率(q)为0.8692,最低平均杂合率(H)为0.1308。同时,用5个微卫星标记对非选育剑尾鱼和近交系RW-H系的基因组进行分析,检测等位基因的个数和大小,结果5个微卫星座位在非选育剑尾鱼中显示为多态,而在近交系RW-H系除1个位点出现等位基因外,其余均为纯合子,计算得到近交系的平均遗传相似系数为0.9345,两种方法均证明近交RW-H系第8代剑尾鱼有较高的遗传纯合度。

关 键 词:剑尾鱼  RW-H近交系  遗传结构  遗传距离  遗传相似系数  实验动物
文章编号:1000-0615(2004)04-0365-06
收稿时间:2014/3/14 0:00:00
修稿时间:2003年1月31日

Genetic structure analysis of Xiphophorus helleri RW-H inbred strain
LI Xia.Genetic structure analysis of Xiphophorus helleri RW-H inbred strain[J].Journal of Fisheries of China,2004,28(4):365-370.
Authors:LI Xia
Abstract:Swordtail fish (Xiphophorus helleri Heckel, 1848) is a kind of small-scaled tropic freshwater fish, which has many characteristics suitable for laboratory animals such as short propagation period, strong reproductive capacity and easy to breed. To date several inbred strains have been obtained by Pearl River Fisheries Research Institute, CAFS. Among the strains, RW - H strain has been inbred into the l lth generation. Researches indicated that this strain is more suitable for genetics, toxicology study and establishment of disease models because of some merits. Genetic purity is one of the most important indices for laboratory animals. So in this paper, RAPD and microsatellite markers were used to analyze the genetic purity of swordtail (Xiphophorus helleri) RW- H inbred strain for directing the breeding of swordtail as laboratory animal. About RAPD, 31 selected single primers were used to amplify genomic DNA of the inbred strain of swordtail. The amplified fragment bands were analyzed to calculate the similarity index and the genetic distance. 383 bands with the lengths between 200bp and 3kb were obtained, among them there were 30 polymorphic bands, the frequency of polymorphic bands was between 0 - 58.33 % and the average was 7.83 %. There was a high frequency of common band in different individuals. Based on RAPD patterns, the calculated similarity index within 11 samples of the inbred strain was 0.9829 (0. 9716- 0. 9960), the mean allelic frequency was 0. 8692, and the minimum heterozygosites was 0. 1308. At the same time, 5 microsatellite markers were used to analyze wild and inbred swordtail genomic DNA. The results showed that the wild was in polymorphism while inbred strain was in monomorphism except for one locus that displayed two alleles. The similarity index of inbred strain calculated from microsatellite analysis was 0. 9345. Both results from RAPD and microsatellite methods indicate that the inbred strain of swordtail RW - H strain has reached a high degree of genetic purity.
Keywords:Xiphophorus helleri  inbred strain  genetic structure  RAPD  microsatellite
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