首页 | 本学科首页   官方微博 | 高级检索  
     检索      

点带石斑鱼的核型、C带、Ag-NORs
引用本文:邹记兴.点带石斑鱼的核型、C带、Ag-NORs[J].水产学报,2005,29(1):33-37.
作者姓名:邹记兴
作者单位:1. 宁波大学生命科学与生物工程学院,浙江,宁波,315211
2. 武汉大学生命科学学院,湖北,武汉,430072
基金项目:浙江省高校青年教师资助计划;宁波大学校内科研基金项目
摘    要:采用PHA活体注射结合秋水仙素培养,取点带石斑鱼全肾,低渗处理,空气干燥制片法制作染色体标本,对染色体进行Giemsa染色、C带及Ag-NORs等系列研究。结果表明:(1)点带石斑鱼2n=48,核型组成为48t,NF=48,没有异型性染色体分化;(2)在最小一对染色体的着丝粒与其染色体臂之间,靠近着丝粒部位有明显的次缢痕;(3)在间期核中,通过银染表现出核仁的数目为1~4个,1个核仁的间期核数目最高,多达55%,4个核仁的间期核数目占2%;(4)50%有丝分裂中期相能观察到Ag-NORs,Ag-NORs主要出现在第24对同源染色体上,第5对同源染色体也可观察到,但其它染色体上则没有;(5)Ag-NORs的数目在不同的细胞中表现出多态性,数目为1~4个,出现4个Ag-NOR~的频率最低(6.8%),出现2个Ag-NORs的频率最高(58.1%);(6)第24对同源染色体近着丝粒的臂内具次缢痕,是A乎NORs所在的区域,该区域分布有大量的结构异染色质,即Ag-NORs与C带强阳性呈现严格的同步对应;(7)点带石斑鱼所有染色体着丝粒为阳性C带,而且第24对染色体几乎整个染色体臂都呈C带阳性,着色强度与该对染色体上的着丝粒C带相同。最后讨论了核型演化规律和Ag-NORs、C带的发生机制。

关 键 词:点带石斑鱼  核型  C带  Ag-NORs
文章编号:1000-0615(2005)01-0033-05
收稿时间:2014/3/13 0:00:00
修稿时间:2003年9月4日

The karyotypes,C-bands patterns and Ag-NORs of Epinephelus malabaricus
ZOU Ji-xing.The karyotypes,C-bands patterns and Ag-NORs of Epinephelus malabaricus[J].Journal of Fisheries of China,2005,29(1):33-37.
Authors:ZOU Ji-xing
Institution:Faculty of Life Science and Biotechnology, Ningbo University, Ningbo315211,China
Abstract:The chromosome specimens of Epinephelus malabaricus (Bloch &Schneider) were obtained from metaphase of kidney cell by viv2injection of PHA and cultivation of colchicines , hypatoic2air drying technique , and then by studying their Giemsa stain , C2bands and Ag2NORs. The results were as follows : 1) E. malabaricus had a diploid chromosome number of 48 and its karyotype formula was 48t , NF = 48 ,sex chromosome was not found;2) There was a pair of chromosomes with secondary constriction close to the centromere of chromosomet24; 3)1-4nucleoliappearedinthenucleusofinterphase,55%nucleihadlnucleolusandonly2%for4nucleoli;4) Ag2NORs appeared in the chromosome t 24 of 50 %metaphase ,sometimes in the chromosome t5 ,but not in other chromosomes ;5) The Ag2NORs polymorphisms were individually specific ,1 -4 pairs of the number ,and the frequency of 4 Ag2NORs were lowest ; 6) The secondary constrictions and positive C2bands were coincident , close centromere the chromosome , and mass constrictive heterochromatinsappearedinthatregion;7)Allthecentromeresofchromoso(tothe) mesweredarkl(of) y stained C2bands ,and the whole arm of chromosome t 24 and its centromere were same positive C2bands. 8) The evolutive regulation of the karyotype and the developing mechanism of Ag2NORs and C2bands were discussed.
Keywords:Epinephelus malabaricus  karyotype  C2bands  Ag2NORs
本文献已被 维普 万方数据 等数据库收录!
点击此处可从《水产学报》浏览原始摘要信息
点击此处可从《水产学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号