饲料中浒苔添加量以及处理方法对幼刺参生长、消化率、消化酶和非特异性免疫酶的影响

采用单因素实验设计,先配制6种浒苔含量(0、5％、10％、15％、20％和25％)饲料饲养初始体质量(1.44±0.01)g幼刺参49 d,然后根据浒苔含量实验结果配制20％含量、3种方法处理的浒苔干燥粉碎(DC)、纤维素酶酶解后干燥粉碎(DCC)和蛋白酶酶解后干燥粉碎(DCP)]饲料饲养初始体质量(4.58 ±0.23)g幼刺参60 d,以研究饲料中浒苔添加量以及处理方式对幼刺参生长、消化率、消化酶和非特异性免疫酶的影响.实验每组饲料设3个重复,每个重复饲喂35头刺参.结果显示:(1)浒苔对提高刺参特定生长率(SGR)、肠道淀粉酶(AMS)活性和超氧化物歧化酶(SOD)和降低饲料系数(FCR)有显著作用(P＜0.05),其中20％浒苔含量最好,浒苔能显著降低饲料干物质表观消化率(ADCd)和粗蛋白质表观消化率(ADCp)(P＜0.05),对刺参摄食率(FI)、成活率(SR)、肠道胃蛋白酶(PP)活性和酸性磷酸酶(ACP)无显著性影响(P＞0.05).(2)3种方法处理的浒苔中,DCC和DCP浒苔对提高刺参SGR、ADCd、ADCp、肠道AMS、PP、SOD和ACP活性和降低FCR有显著作用(P＜0.05),其中DCC浒苔最好,3种方法处理的浒苔对刺参FI和SR亦无显著性影响(P＞0.05).在本实验条件下,幼刺参饲料中浒苔适宜含量为20％;纤维素酶酶解后干燥粉碎浒苔是一个理想的浒苔处理方法.

Effects of dosage and treatments of Enteromorpha prolifera on growth, digestibility,digestive enzymes and non-specific immunity enzymes of juvenile sea cucumber(Apostichopus japonicus Selenka)

The experiment was conducted to investigate the effects of dosage and treatments of Enteromorpha prolifera on growth, digestibility, digestive enzymes and non-specific immunity enzymes of juvenile sea cucumber(Apostichopus japonicus Selenka).Juvenile sea cucumbers with the initial weight of(1.44±0.01)g were fed with 6 practical diets according to a single factor design:a basal diet contained 0, 5%, 10%, 15%, 20% and 25% E.prolifera for 49 days firstly, and then juvenile sea cucumber with the initial weight of(4.58±0.23)g were fed with 3 practical diets according to a single factor design:the diet contained 20% three treatmentsdry crushing(DC), dry crushing after cellulase treatment(DCC)and dry crushing after protease treatment(DCP)]disposal(Wrong word)of E.proliferat for 60 days.There were 3 replicates per group with 35 sea cucumbers.The experiment results showed as follows:1)E.prolifera had significant influence on enhancing specific growth rate(SGR), the amylase(AMS)and the superoxide dismutase(SOD)in gut of sea cucumber and reducing feed conversion ratio(FCR)(P<0.05), and 20% E.prolifera were the best.The apparent digestibility coefficient of dry matter(ADCd)and apparent digestibility coefficient of crude protein(ADCp)of diets were significantly declined with the increasing level of E.prolifera(P<0.05).The different contents of E.prolifera had no significance on feed intake(FI), survival rate(SR), the pepsin(PP)and acid phosphatase(ACP)in gut of sea cucumber(P>0.05).2)E.prolifera with DCC and DCP had significant influence on enhancing SGR, ADCd, ADCp, AMS, PP, SOD and ACP in gut of sea cucumber and reducing FCR of sea cucumbers(P<0.05), and E.prolifera with DCC were the best.Three treatment of E.prolifera had no significant influence on FI and SR(P>0.05).Under the conditions of this experiment, the optimal content of E.prolifera in the diets of juvenile sea cucumber is 20%.And E.prolifera with drying crush after cellulase treatment is an ideal way to deal with E.prolifera.