副溶血弧菌对斑节对虾非特异性免疫酶活性的影响

为了研究副溶血弧菌对斑节对虾非特异性免疫酶活性的影响,分别对斑节对虾注射生理盐水和副溶血弧菌,于不同时间点测定肝胰腺和鳃中的总抗氧化能力(T-AOC)、酸性磷酸酶(ACP)、碱性磷酸酶(ALP)和溶菌酶(LSZ)的活性变化。结果显示,与对照组相比,感染副溶血弧菌后,肝胰腺和鳃中T-AOC活性分别于12和6 h达到最大值(P0.05),随后逐渐降低;肝胰腺中ACP活性于3 h显著升高,随后逐渐降低,并于24 h达到最小值(P0.05),而鳃中ACP活性于12 h达到最大值,随后逐渐降低,并于48 h显著低于对照组(P0.05);肝胰腺和鳃中ALP活性整体均呈现出先升高后下降的趋势,但未出现显著低于对照组的现象;肝胰腺和鳃中LSZ活性均于3 h显著升高至最大值(P0.05),随后逐渐恢复至初始水平。研究表明,副溶血弧菌感染对斑节对虾非特异性免疫酶活有显著影响,对其机体免疫防御系统有明显的破坏作用。

The non-specific immune response of Penaeus monodon to Vibrio parahaemolyticus challenge

To study the effect of Vibrio parahaemolyticus challenge on the non-specific immune function of Penaeus monodon,healthy P.monodonaverage body weight(5.65±0.34)g]were used to determine the changes of non-specific immune enzyme activities in hepatopancreas and gill tissues after being injected with sterile 0.86% normal saline and V.parahaemolyticus.Activities of total antioxidant capacity(T-AOC),acid phosphatase(ACP),alkaline phosphatase(ALP)and lysozyme(LSZ)were measured at different sampling time points.The results showed that:compared with the control group,after being challenged with V.parahaemolyticus,T-AOC activities in hepatopancreas and gill were both increased to the peak value at 12 and 6 h(P<0.05),respectively,and then decreased gradually.ACP activities in hepatopancreas were up-regulated significantly at 3 h(P<0.05),then decreased gradually and reached the lowest level at 24 h(P<0.05).In gill,ACP activities were increased to the maximum at 12 h,and then decreased gradually to a lower level than the control at 48 h(P<0.05).ALP activities in hepatopancreas and gill both showed a downward tendency after rising first in general,but did not exhibit the phenomenon of significantly lower than the control group.LSZ activities in hepatopancreas and gill both increased significantly to the peak level at 3 h,and then returned to the control group level gradually.The results indicated that V.parahaemolyticus challenge had noticeable impact on non-specific immune enzyme activities of P.monodon,and significant destructive effect to the shrimp's immune system.