| 中华绒螯蟹促性腺释放激素类似物的初步分离纯化与免疫鉴定 |
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| 引用本文： | 王承志,邱高峰.中华绒螯蟹促性腺释放激素类似物的初步分离纯化与免疫鉴定[J].水产学报,2012,36(6):908-913. |
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| 作者姓名： | 王承志 邱高峰 |
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| 作者单位： | 上海海洋大学农业部水产种质资源与利用重点开放实验室,上海,201306 |
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| 基金项目： | 国家自然科学基金项目(30972242); 国家教育部博士点科研基金项目(20093104110003); 上海市科委重点科研项目(09391911100); 上海市教育委员会海洋生物学重点学科建设项目(J50701) |
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| 摘 要： | 应用反向高效液相色谱法(RP-HPLC)从中华绒螯蟹脑组织提取液初步分离了促性腺释放激素(GnRH)类似物,使用章鱼促性腺释放激素抗体(anti-octGnRH)对分离组分进行免疫斑点印迹实验鉴定,获得了免疫反应呈阳性分离组分,表明脑组织粗提液中含有GnRH类似物分离组分。对该GnRH类似物进行基质辅助激光解吸/电离串联飞行时间质谱(MALDI-TOF/TOFMS)分析,结果显示多肽物质分子量范围在800～1400u,主要由8种小分子多肽组成,其中一种组分的分子量与章鱼促性腺释放激素(octGnRH)分子量接近。
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| 关 键 词： | 中华绒螯蟹 反向高效液相色谱法 免疫斑点印迹 促性腺释放激素 |
| 收稿时间： | 12/5/2011 5:46:48 PM |
| 修稿时间： | 2/10/2012 9:30:14 AM |
Partial isolation and immunological characterization of a GnRH-like peptide from Chinese mitten crab (Eriocheir sinensis) |
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| WANG Cheng-zhi and QIU Gao-feng.Partial isolation and immunological characterization of a GnRH-like peptide from Chinese mitten crab (Eriocheir sinensis)[J].Journal of Fisheries of China,2012,36(6):908-913. |
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| Authors: | WANG Cheng-zhi and QIU Gao-feng |
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| Institution: | Key Laboratory of Aquatic Genetic Resources and Utilization Certificated by Ministry of Agriculture,Shanghai Ocean University,Key Laboratory of Aquatic Genetic Resources and Utilization Certificated by Ministry of Agriculture,Shanghai Ocean University |
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| Abstract: | In this study a gonadotropin-releasing hormone (GnRH)-like peptide was partially isolated by reverse phase high performance liquid chromatography (RP-HPLC) from the brain extract of Chinese mitten crab Eriocheir sinensis. Immunological dot blot analyses from RP-HPLC fractions using the anti-octGnRH revealed that one of fractions is immune reaction positive, suggesting that the fractions of brain extract contain GnRH-like substance. Eight kinds of small peptides, with molecular weight ranging from 800 to 1 400 u, were further identified in this fraction by using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS). One of peptides in MS component shared similar molecular weight with the octGnRH. |
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| Keywords: | Eriocheir sinensis reversed phase high performance liquid chromatography immunological dot blot gonadotropin-releasing hormone |
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