| 鳜鱼病毒核酸的初步分析 |
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| 引用本文： | 李新辉.鳜鱼病毒核酸的初步分析[J].水产学报,2000,24(2):171-174. |
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| 作者姓名： | 李新辉 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所,广州,510380 |
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| 基金项目： | 农业部资助，广东省科委资助，广东省自然科学基金资助! (鳜鱼病毒核酸随机表达库建立及抗原基因筛选与表达研究 ) ,980 90 7号 |
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| 摘 要： | 提取鳜鱼病毒核酸，分别用ＤＮａｓｅ、ＲＮａｓｅ和绿豆芽核酸酸处理表明，该病毒为双链ＤＮＡ病毒，用带ＥｃｏＲⅠ酶切位点的随机引物扩增病毒核酸，获得扩增核酸带谱。经低熔点琼脂糖回收ＰＣＲ产物，与质粒ｐＵＣ１９连接，获得三个重组子，已经对两个小插入片断进行了序列分析，插入序列分别为３６９ｂｐ和４５０ｂｐ。ＧｅｎＢａｎｋ检测显示，尚未有类似的序列报导。
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| 关 键 词： | 鳜鱼病毒 核酸 克隆 序列 检测 |
| 收稿时间： | 2014/4/17 0:00:00 |
| 修稿时间： | 1999-03-26 |
Primary analysis for nucleic acid of Siniperca chuatsi virus |
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| Li Xinhui.Primary analysis for nucleic acid of Siniperca chuatsi virus[J].Journal of Fisheries of China,2000,24(2):171-174. |
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| Authors: | Li Xinhui |
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| Institution: | Pearl River Fisheries Research Institute,CAFS, Guangzhou 510380 |
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| Abstract: | The virus nucleic acid was isolated from disease Siniperca chuatsi , and extracted with RNase,
DNase and Mung bean nuclease respectively. The tests indicated that the virus genome is dsDNA. Part of the
genome of Siniperca chuatsi virus were successful amplif ied by random primer ( contained the enzyme position of
EcoR .. ) . The PCR products were recovered from low melting-temperature agarose and cloned in pUC19 plasmid.
Three kinds of recombinant plasmids have been identified with EcoR ... Two kinds of it have been sequenced.
There were 369bp and 450bp in size. It was demonstrated that there were not homology sequences against
tho se in GenBank. |
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| Keywords: | Siniperca chuatsi virus nuleic acid clone sequence |
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