| 日本绒螯蟹ND2基因全序列测定与分析 |
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| 引用本文： | 柳广东.日本绒螯蟹ND2基因全序列测定与分析[J].水产学报,2005,29(5):711-714. |
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| 作者姓名： | 柳广东 |
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| 作者单位： | 1. 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003
2. 东京水产大学水生生物系,日本,东京,108-8477 |
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| 基金项目： | 中国海洋大学海水养殖教育部重点实验室开放课题（200411） |
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| 摘 要： | 动物线粒体DNA（mtDNA）具有比核DNA的进化速率快等特点，而被广泛地应用于生物的起源、演化和种群遗传学研究。完整的线粒体基因组包括37个基因：13个编码疏水性蛋白质亚基基因、22个tRNA基因、2个rRNA基因。各基因排列紧密，不含内含子，且有些基因区域出现重叠。
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| 关 键 词： | 日本绒螯蟹 ND2基因 全序列 |
| 文章编号： | 1000-0615（2005）05-0711-04 |
| 收稿时间： | 2004-07-06 |
| 修稿时间： | 2004年7月6日 |
The complete sequence of the mitochondrial ND2 gene of Eriocheir japonica |
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| LIU Guang-dong.The complete sequence of the mitochondrial ND2 gene of Eriocheir japonica[J].Journal of Fisheries of China,2005,29(5):711-714. |
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| Authors: | LIU Guang-dong |
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| Institution: | 1. The Key Ladoratory of Mariculture, Ministry of Education, Ocean. University of China, Qingdao 266003, China ; 2. Department of Aquatic Bioscieuces, Tokyo University of Fisheries, Tokyo 108 - 8477, Japan |
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| Abstract: | Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2(ND2) gene in mitochondrial tRNA~(Met) and tRNA~(Trp) gene respectively,and the complete sequence of the ND2 gene of Eriocheir japonica was determined.The results indicated that the length of ND2 gene of Eriocheir japonica was 1009 bp.The A,T,G,C contents of the gene were 28.54%(288 bp),44.20%(446 bp),8.23%(83 bp),and 19.03%(192 bp) respectively;and the sequence was AT rich and biased.The start and stop codons were ATC and A respectively.The homology analysis of nucleotide sequence showed that the similarities in ND2 gene between Eriocheir japonica and other crustacean were comparatively low,which suggested that ND2 gene could be effective molecular marker for further study on phylogeny of Eriocheir species. |
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| Keywords: | Eriocheir japonica ND2 gene complete sequence |
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