尿囊素对印度产紫菜Pyropia chauhanii单孢子放散和体细胞分化的影响

为探讨尿囊素对印度产紫菜Pyropia chauhanii单孢子形成与放散的影响,选用能释放单孢子的野生型品系(PC-WT)与不能释放单孢子的诱变品系(PC-Y1)为实验材料,用含不同浓度尿囊素的培养液分别培养来自叶状体梢、中和基部的圆盘体。结果显示,处理后6 d,不同部位的叶状体圆盘体的单孢子放散量次序为中部梢部基部;随着尿囊素浓度的增加,PC-WT品系叶状体圆盘体的单孢子放散总量呈先升后降的趋势,其中10mmol/L是促进单孢子放散的最适浓度;但尿囊素并不能使原本不放散单孢子的PC-Y1品系释放单孢子。用含20 mmol/L尿囊素的培养液培养PC-Y1品系的叶状体圆盘体,8 d后再用酶解法将其体细胞单离出来经体外培养后发现,经尿囊素处理的单离细胞发育成丝状体的百分率为66.1%,而不含尿囊素的对照组仅为15.2%,说明尿囊素对紫菜叶状体的体细胞向生殖细胞分化具一定的促进作用。

The influence of allantoin on monospores release and somatic cell differentiation in gametophytic blades of Pyropia chauhanii

In order to explore the influence of allantoin on monospores formation and release of Pyropia chauhanii, we used the wild-type strain (PC-WT) which could release monospore and its induced strain (PC-Y1) which could not release monospore as materials, then different strains blade discs of apical, middle and basal sections were cultured in medium adjusted with different concentration of allantoin. The research results showed that, in 6 days after treatment, the order of the release number of the different parts of each disc was middle>apical>basal; With the increasing of allantoin concentration, the numbers of release monospores of PC-WT strain blade discs showed a trend of increasing first and then decreasing, and 10 mmol/L was the optimal allantoin concentration of promoting monospore release. But allantoin could not promote PC-Y1 release monospore. We used 20 mmol/L allantoin of medium to culture the discs of PC-Y1 blade for 8 days, and single somatic cell was obtained using enzymatic hydrolysis from the discs of different section, and continue culturing in vitro. We found that the percentage of single cells developed into conchocelis was 66.1% in treatment group; and the percentage of single cells developed into conchocelis was only 15.2% in control group. The result showed that allantoin could promote differentiation from somatic cell to germ cell of blade in Pyropia.