江黄颡鱼卵细胞膜的结构特征

Using conventional histochemical methods and scanning electron microscope, the egg surface of mature oocyte of Pelteobagrus vachelli was observed. The micropyle is funnel-like and consists of micropylar vestibule and micropylar canal. The opening of micropylar vestibule is about 11.35μm in diameter while entrance of the micropyle to the oocyte is only about 2.6 μm. There are plenty of micropores on the surface near the micropyle. Micropyle might act as an apparatus leading spermatozoa to penetrating an oocyte. Regular cord-like structures between basal membrane (the outermost layer of egg surface) and plasmic membrane (the inner layer of egg surface) are found. These cord-like structures, each about 16.75 to 18.50μm in length, 5μm in diameter, comprised small balls whose diameters vary from 2.18 to 2.5μm and are arranged cornpactly under the basal membrane. These kinds of structures are zona radiata and act as special eggshell. After activation of the oocytes, the cord-like structures would partially transform into mucoprotein. To our best knowledge, this paper is the fast report on stereo view of the zona radiata of teleost oocyte.

Structural characteristics of egg surface of Pelteobagrus vachelli

Using conventional histochemical methods and scanning electron micro scope, the egg surface of mature oocyte of Pelteobagrus vachelli was observed. The micropyle is funne-l like and consists of micropylar vestibule and micropylar canal. The opening of micropylar vestibule is about 11. 35 Lm in diameter while entrance of the micropyle to the oocyte is only about 2. 6 Lm. There are plenty of micropores on the surface near the micropyle. Micropyle might act as an apparatus leading spermatozoa to penetrating an oocyte. Regular cord- like structures between basal membrane ( the outermost layer of egg surface) and plasmic membrane ( the inner layer of eg g surface) are found. These cord- like structures, each abo ut 16. 75 to 18. 50 Lm in length, 5 Lm in diameter , comprised small balls whose diameters vary from 2. 18 to 2. 5 Lm and are arranged compactly under the basal membrane. These kinds of structures are zona radiata and act as special eg gshell. After activation of the oocytes, the cord- like structures would partially transform into mucoprotein. To our best knowledge, this paper is the first report on stereo view of the zona radiata of teleost o ocyte.