急性铅染毒对河南华溪蟹副性腺诱导精子顶体反应的影响

为探究河南华溪蟹副性腺的功能以及铅对副性腺功能的影响和毒性机理,首先采用钙离子载体A23187诱导法对河南华溪蟹进行精子顶体反应诱导实验,结果显示副性腺匀浆+A23187组合下的顶体反应诱导率最高,可达78%,这提示河南华溪蟹副性腺具有调节精子顶体反应的作用。在此基础上采用急性铅染毒的方法,设1个对照组和5个铅处理组(3.675、7.35、14.70、29.40和58.80 mg/L),染毒时间分别为3、5和7 d,检测了铅染毒后的副性腺对精子顶体反应诱导率的影响,以及染毒后副性腺中活性氧(ROS)含量、蛋白质羰基化(PCO)含量、DNA-蛋白质交联率(DPC)的变化。结果显示,随着铅染毒剂量的增加和染毒时间的延长,精子顶体反应诱导率明显下降,副性腺中ROS含量和PCO含量升高,但DPC无明显变化。研究表明,铅对河南华溪蟹副性腺有明显的生殖毒性,可降低其在精子顶体反应诱导过程中的作用,主要原因是铅诱导产生的ROS使副性腺中蛋白质发生了氧化损伤。

The effect of acute lead on accessory gland of freshwater crab (Sinopotamon henanense) in sperm acrosome reaction induction

To investigate the toxic effect of lead on male accessory gland function in the freshwater crab Sinopotamon henanense, the function of accessory gland was investigated firstly, acrosome reaction induction rate was high up to 78% under the combination of calcium ionophore A23187 and accessory gland homogenate, it implied that accessory gland could adjust the action of sperm acrosome reaction. Then the effects of Pb on the accessory gland function in sperm acrosome reaction induction and oxidative stress were investigated after 3, 5 and 7 d, exposed to different Pb concentrations (0, 3.675, 7.35, 14.70, 29.40 and 58.80 mg/L). The results showed that the acrosome reaction induction rate was deceased significantly when the accessory gland was taken from crabs which was treated by Pb. Meanwhile, the reactive oxygen species (ROS) content and protein carbonylation (PCO) content were increased in a concentration- and time-dependent manner in accessory gland, however, DNA-protein crosslinks rate (DPC) level had no significant change. Our findings documented that Pb deceased the function of accessory gland in sperm acrosome reaction induction, which was associated with the increasing of ROS leading to protein oxidative damage.