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我国六个地区银鲫种群线粒体DNA多态性的研究
引用本文:姚纪花.我国六个地区银鲫种群线粒体DNA多态性的研究[J].水产学报,1998,22(4):289-295.
作者姓名:姚纪花
作者单位:上海水产大学渔业学院
基金项目:“九五”农业部重点科研项目
摘    要:本文用一对引物对我国6个地区银鲫进行mtDNA-ND5/6片段的PCR扩增,扩增产物用6种限制性内切酶进行限制生片段长度多态性分析。共获得11种图谱和7种限制性类型。根据各群体间的遗传距离构建了UPG聚类关系图。结果表明,6个银鲫种群中,云南群体与贵州群体的关系以及黑龙江与安徽群体之间的关系最近,它们分别归为一个群体;河南群体相对独立,它与黑龙江和安徽群体的遗传关系较近,江西群体与各群体间的亲缘关

关 键 词:银鲫  种群  线粒体DNA  限制性片段长度  多态性
收稿时间:5/6/2014 12:00:00 AM
修稿时间:5/6/2014 12:00:00 AM

A STUDY ON MITOCHONDRIAL DNA POLYMORPHIS AMONG SIX GEOGRAPHICAL POPULATIONSOF CARASSIUS AURATUS GIBELIO IN CHINA
YAO Ji Hua.A STUDY ON MITOCHONDRIAL DNA POLYMORPHIS AMONG SIX GEOGRAPHICAL POPULATIONSOF CARASSIUS AURATUS GIBELIO IN CHINA[J].Journal of Fisheries of China,1998,22(4):289-295.
Authors:YAO Ji Hua
Institution:Fisheries College,Shanghai Fisheries University, 200090
Abstract:In six geographical populations of Carassius auratus gibelio, the ND 5/6 region (approximately 2.5Kb) of mtDNA genome have been amplified by the polymerase chain reaction (PCR), and the products were examined using restriction fragment length polymorphism (RFLP). 11 electrophoresis patterns and 7 haplotypes (genotypes) have been observed. Based on the genetic distances, the UPG molecular phylogenetic tree of the populations was constructed. The results indicate that the six populations of the C. auratus gibelio can be imcorporated into four populations, namely, the Heilongjiang and Anhui, the Henan, the Yunnan and Guizhou, and the Jiangxi.The first is the closest to the third. The Henan is much closer to the Heilongjiang and Anhui. The farthest is between the Jiangxi and the other populations.
Keywords:Carassius auratus gibelio  Population    mtDNA  RFLP  
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