鳗鲡疱疹病毒的生物学及理化特性

为了明确鳗鲡疱疹病毒(Anguillid herpesvirus, AngHV)的生物学及理化特性,本实验利用一株从欧洲鳗鲡"脱黏败血综合征"病料中分离的AngHV病毒株,研究了其增殖特性及其对主要鱼类细胞系的感染敏感性,进一步分析了其对热、酸碱、氯仿和乙醚等理化因子的耐受性。结果发现,AngHV感染的鳗鲡卵巢细胞系(eel ovary cell line, EO)内可见典型的疱疹病毒样颗粒,细胞出现时序性细胞病变;AngHV可在EO细胞系内稳定传代,较适宜扩繁温度为25～27°C,不能在鲤上皮瘤细胞系(epithelioma papilloma cyprinid cell line, EPC)、草鱼卵巢细胞系(grass carp ovary cell line, CO)、胖头逓肌肉细胞系(fathead minnow cell line, FHM)、大鳞大麻哈鱼胚胎细胞系(chinook salmon embryo cell line,CHSE-214)、虹鳟性腺细胞系(rainbow trout gonad cell line, RTG-2)及蓝鳃太阳鱼细胞系(bluegill fry cell line, BF-2)等鱼类细胞内增殖;理化特性分析表明,37°C处理30 min,AngHV滴度降低不明显,而56°C处理30 min可完全灭活AngHV;AngHV对酸(pH 3.0)敏感,而对碱(pH 10.0)较耐受,同时对氯仿和乙醚敏感。本研究结果可为AngHV的综合防控及疫苗的开发提供参考依据。

Biological and physicochemical characteristics of Anguillid herpesvirus

To clarify the biological and physicochemical properties of Anguillid herpesvirus (AngHV), an AngHV strain, NA16108, isolated from the diseased Anguilla anguilla of "mucus sloughing and hemorrhagic septicemia disease" was used to study its replication characteristics and infection sensitivity to some fish continuous cell lines, and its survivability to heat, acid, alkali, chloroform, ether and other physical and chemical factors was further analyzed. The results indicated that AngHV infected eel ovary cells (EO) showed typical sequential cytopathic effects (CPE), and many typical herpesvirus-like viral particles could be seen in the infected cells. AngHV could propagate stably in EO cells, and the suitable temperature for propagation is 25 °C to 27 °C, but it could not propagate in epithelioma papilloma cyprinid cells (EPC), grass carp ovary cells (CO), fathead minnow cells (FHM), chinook salmon embryo cells (CHSE-214), rainbow trout gonad cells (RTG-2), bluegill fry cells (BF-2). The titer of AngHV slightly decreased after 30 min treatment at 37 °C, and it could be completely inactivated after 30 min treatment at 56 °C. AngHV is sensitive to acid (pH 3.0), but resistant to alkali (pH 10.0), and highly sensitive to chloroform, ether and other organic solvents. These results can provide references for the prevention and control of AngHV diseases.