| 饲料中糖/脂肪比对暗纹东方鲀幼鱼生长、血液指标、肝代谢酶活性及PEPCK基因表达的影响 |
| |
| 引用本文： | 刘襄河,叶超霞,沈碧端,王春艳,彭键,王安利.饲料中糖/脂肪比对暗纹东方鲀幼鱼生长、血液指标、肝代谢酶活性及PEPCK基因表达的影响[J].水产学报,2014,38(8):1149-1158. |
| |
| 作者姓名： | 刘襄河 叶超霞 沈碧端 王春艳 彭键 王安利 |
| |
| 作者单位： | 华南师范大学生命科学学院,华南师范大学生命科学学院,华南师范大学生命科学学院,华南师范大学生命科学学院,华南师范大学生命科学学院,华南师范大学生命科学学院 |
| |
| 基金项目： | 广东省重点实验室建设项目(2009A060800019)；省部产学研结合项目(2011B090500009)；广东省高等学校科技创新重点项目(CXZD1114)。 |
| |
| 摘 要： | 配制6种不同糖/脂肪比例（CHO∶L分别为4.29、2.79、1.86、1.19、0.73和0.41）的等氮等能饲料，饲养暗纹东方鲀幼鱼初始体重为(11.2 ± 0.5) g] 60 d，探讨饲料中糖与脂肪比例对其生长、饲料利用、生理生化指标和PEPCK基因表达的影响。试验结果表明：随饲料中CHO∶L降低，暗纹东方鲀特定生长率（SGR）、蛋白质效率（PER）和饲料效率（FE）先升高后降低，且均在CHO∶L为1.86时达最大，显著高于CHO∶L为0.41、0.73和4.29时的值(P<0.05)。血浆甘油三酯和肝脏脂肪含量随CHO∶L降低而显著增加(P<0.05)，而血糖和肝糖原含量随CHO∶L降低而显著降低(P<0.05)。随饲料中CHO∶L降低，肝脏脂肪酶和脂肪合成酶活性先增加后降低，分别在CHO∶L为1.19和1.86时活性最高。CHO∶L为1.86-4.29时PK活性显著高于其他饲料组(P<0.05)。CHO：L为0.41和0.73时，AMS活性显著低于其他饲料组(P<0.05)，而PEPCK活性和mRNA相对表达量显著高于其他饲料组(P<0.05)。饲料中CHO∶L对暗纹东方鲀存活率和血浆总胆固醇含量无显著影响(P>0.05)。分别用二次多项回归模型拟合SGR、PER、FE和CHO∶L的关系，得到暗纹东方鲀幼鱼饲料中CHO∶L的适宜范围为2.01~2.16，且其对碳水化合物的利用能力要高于脂肪。
|
| 关 键 词： | 暗纹东方鲀 糖脂比 生化指标 PEPCK基因 |
| 收稿时间： | 2014/3/12 0:00:00 |
| 修稿时间： | 2014/5/12 0:00:00 |
Effect of dietary carbohydrate-to-lipid ratio on growth, blood biochemical indices,hepatic metabolic enzymes and PEPCK gene expression of juvenile obscure puffer(Takifugu obscurus) |
| |
| LIU Xianghe,YE Chaoxi,SHEN Biduan,WANG Chunyan,PENG Jian and WANG Anli.Effect of dietary carbohydrate-to-lipid ratio on growth, blood biochemical indices,hepatic metabolic enzymes and PEPCK gene expression of juvenile obscure puffer(Takifugu obscurus)[J].Journal of Fisheries of China,2014,38(8):1149-1158. |
| |
| Authors: | LIU Xianghe YE Chaoxi SHEN Biduan WANG Chunyan PENG Jian and WANG Anli |
| |
| Institution: | Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University |
| |
| Abstract: | This experiment was conducted to evaluate the effects of dietary carbohydrate-to-lipid ratio on growth performance, feed utilization, biochemical indices and phosphoenolpyruvate carboxykinase(PEPCK)gene expression in juvenile obscure puffer, Takifugu obscurus.Six isonitrogenous and isoenergetic diets were formulated with dietary CHO:L ratios of 4.29, 2.79, 1.86, 1.19, 0.73 and 0.42, respectively.Fish(initial weight 11.2±0.5 g)were stocked in triplicate tanks(25 fish per tank)and fed to satiation during a period of 60 days.The results indicated that best specific growth rate, protein efficiency ratio and feed efficiency were found in fish fed diet with CHO:L of 1.86, which were significantly higher than those of fish fed diets with 0.42, 0.73 and 4.29(P<0.05).Plasma triglyceride and liver lipid content increased significantly as dietary CHO:L ratio decreasing(P<0.05), while plasma glucose and hepatic glycogen content showed a contrary tendency.Activities of lipase, fatty acid synthase, amylase and pyruvate kinase in liver were significantly affected by dietary CHO:L ratio(P<0.05).Fish fed diets with CHO:L of 0.42 and 0.73 had significantly higher PEPCK activity and mRNA relative expression than those fed with the other diets(P<0.05).However, survival and plasma total cholesterol content were unaffected by dietary CHO:L ratio.The optimal range of CHO:L within 2.01-2.16 is suitable for juvenile obscure puffer based on second-order polynomial regression analysis of SGR, PER and FE against dietary CHO:L ratio, respectively. |
| |
| Keywords: | Takifugu obscurus carbohydrate-to-lipid ratio biochemical indice |
|
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
