| 可溶性南极磷虾蛋白-芦丁复合物的制备及抗氧化性能分析 |
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| 引用本文： | 李玉锋,王敬敬,檀利军,彭知云,赵晟,刘海泉,赵勇.可溶性南极磷虾蛋白-芦丁复合物的制备及抗氧化性能分析[J].水产学报,2022,46(3):475-485. |
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| 作者姓名： | 李玉锋 王敬敬 檀利军 彭知云 赵晟 刘海泉 赵勇 |
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| 作者单位： | 安徽工程大学生物与食品工程学院,佛山科学技术学院食品科学与工程学院,上海海洋大学食品学院,上海海洋大学食品学院,上海海洋大学食品学院,上海海洋大学食品学院,上海海洋大学食品学院 |
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| 基金项目： | 国家重点研发计划（2018YFC1602205）；安徽工程大学引进人才科研启动基金（2021YQQ046） |
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| 摘 要： | 为了提升可溶性南极磷虾蛋白的抗氧化性能,将芦丁复合到可溶性南极磷虾蛋白(SAKP)上,成功制备了SAKP-芦丁复合物,一种新型的多酚-蛋白复合物.通过测定SAKP-芦丁复合物清除二苯基苦基苯肼自由基(DPPH?)和羟基自由基(OH?)的能力来评估其抗氧化能力.同时采用H2O2诱导HepG-2细胞氧化损伤模型评价了SAK...
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| 关 键 词： | 可溶性南极磷虾蛋白 芦丁 复合物 抗氧化 HepG-2细胞 |
| 收稿时间： | 2021/11/9 0:00:00 |
| 修稿时间： | 2021/12/27 0:00:00 |
Preparation and antioxidant properties of soluble Antarctic krill protein-rutin complexes |
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| LI Yufeng,WANG Jingjing,TAN Lijun,PENG Zhiyun,ZHAO Cheng,LIU Haiquan,ZHAO Yong.Preparation and antioxidant properties of soluble Antarctic krill protein-rutin complexes[J].Journal of Fisheries of China,2022,46(3):475-485. |
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| Authors: | LI Yufeng WANG Jingjing TAN Lijun PENG Zhiyun ZHAO Cheng LIU Haiquan ZHAO Yong |
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| Institution: | Anhui Polytechnic University,chool of Food Science and Engineering, Foshan University,,Shanghai Ocean University,,,College of Food Sciences and Technology, Shanghai Ocean University |
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| Abstract: | In this work, for improving the antioxidant activity of Antarctic krill protein, a new polyphenol-protein complexes (SAKP-rutin complexes) was successfully prepared by grafting rutin onto soluble Antarctic krill proteins (SAKP). The antioxidant capacity of SAKP-rutin complexes was evaluated by measuring its ability to scavenge DPPH- and hydroxyl radicals. Meanwhile, the protective ability of SAKP-rutin complexes against oxidative damage of HepG-2 cells was determined by using a H2O2 induced oxidative damage model. The results showed that the combination of rutin greatly enhanced the ability of SAKP to scavenging free radicals (p<0.05). Compared with natural SAKP, the SAKP-rutin complexes significantly increased the activity of cellular antioxidant enzymes (CAT, SOD and GSH), decreased the production of lipid peroxidation products (MDA) in cells caused by oxidative stress, and finally reduced the damage of oxidative stress to cells. These findings suggest that SAKP-rutin complexes are a novel protein resource which is hopefully applied to developing functional protein food. |
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| Keywords: | soluble Antarctic krill proteins rutin complex antioxidant HepG-2 cell |
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