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金属硫蛋白基因对转基因鲤及实验动物体内重金属累积的影响
引用本文:闫学春.金属硫蛋白基因对转基因鲤及实验动物体内重金属累积的影响[J].水产学报,2005,29(2):281-284.
作者姓名:闫学春
作者单位:中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070
基金项目:国家863项目资助(863-101-05-02-01)
摘    要:金属硫蛋白(metallothiontine,简称MT),又称重金属结合蛋白,是一种低分子量富含半胱氨酸的金属结合蛋白。锌、铜、镉等多种金属元素均可在体外或体内诱导组织MT大量合成。已有研究表明,MT在微量元素代谢、重金属富集、环境污染的清除等方面起重要作用,因此, 选择恰当的启动子是保证外源基因在转基因鱼中成功表达的最重要因素。在转基因鱼的研究过程中,使用的转基因材料从最初阶段的人及其它哺乳类如牛、羊等的生长激素基因发展到现在的鱼的生长激素基因,启动调控顺序也由小鼠的重金属螯合蛋白(MT-1)基因发展到鱼的重金属结合蛋白基因,如虹鳟金属硫蛋白基因、鲤β-肌动蛋白基因、美洲鲽抗冻蛋白基因以及鲤金属硫蛋白

关 键 词:金属硫蛋白基因  转基因鲤  实验动物  重金属累积
文章编号:1000-0615(2005)02-0281-04
收稿时间:2014/3/14 0:00:00
修稿时间:2003年8月1日

The effect of MT gene on heavy metal accumulations in transgenic common carp and experimental animal
YAN Xue-chun.The effect of MT gene on heavy metal accumulations in transgenic common carp and experimental animal[J].Journal of Fisheries of China,2005,29(2):281-284.
Authors:YAN Xue-chun
Institution:Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070,China
Abstract:One of the most important things for industry production of transgenic fish is whether the fish meal is safe to the health of human beings, which means the foreign genes and their expressed products might be harmful to fish and the animal eating this fish. For the transgenic common carp produced by injecting fusion genes which included chum salmon's growth hormone gene and common carp's metallothionine promoter, two things should be investigated. One is whether the transgenic common carp will produce excessive amounts of growth hormone in its body, and the foreign hormone how to affect the animal eating this fish. The other thing is whether this fish will accumulate excessive heavy metal in its body, what effect will be on the animal eating this fish. The investigation of the concentrations of the foreign growth hormone in transgenic carp is reported. This article examines the effect of a metallothionine transgene on the residual heavy metal concentrations in the transgenic common carp and the cat eating the transgenic carp. Using flame atomic absorption spectrophotometry, we measured the concentrations of the heavy mental elements including Zn, Cu,Cd,and Pb in the muscle,liver,kidney and gills of the transgenic common carp and non-transgenic carp. Also measured were the concentrations of Zn and Cd in the muscle,liver and kidney of the cat fed with transgenic and non-transgenic common carps. In the cat, there was no significant difference (P > 0.05) in tissue concentrations of heavy metals between the experimental group and the control group. The heavy metal concentrations in the non-transgenic common carp and transgenic common carp were statistically similar. The result showed that metallothionine gene as a promoter for aquaculture fish genetransfer would not bring about the heavy metal accumulations in transgenic fish and animal eating them.
Keywords:metallothiontine gene(MT gene)  transgenic common carp  experimental animal  heavy metal accumulation
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