| 基于ND4和ND5基因序列研究八种臂尾轮虫的系统关系和分类地位 |
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| 引用本文： | 程双怀,魏世祥,张逸,孙道琴,江静,穆维平,席贻龙,葛雅丽.基于ND4和ND5基因序列研究八种臂尾轮虫的系统关系和分类地位[J].水产学报,2016,40(12):1823-1829. |
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| 作者姓名： | 程双怀 魏世祥 张逸 孙道琴 江静 穆维平 席贻龙 葛雅丽 |
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| 作者单位： | 1. 安徽师范大学生命科学学院,安徽芜湖241000;安徽省高校生物环境与生态安全省级重点实验室,安徽芜湖241000;安徽省重要生物资源保护与利用研究省级重点实验室,安徽芜湖241000;2. 安徽师范大学生命科学学院,安徽芜湖,241000 |
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| 基金项目： | 国家自然科学基金；安徽省自然科学基金； |
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| 摘 要： | 通过对尾突臂尾轮虫、裂足臂尾轮虫、剪形臂尾轮虫、方形臂尾轮虫、红臂尾轮虫、镰形臂尾轮虫、矩形臂尾轮虫和十指臂尾轮虫8种臂尾轮虫的ND4和ND5基因序列进行扩增、测序,结合Gen Bank数据库中的褶皱臂尾轮虫、萼花臂尾轮虫、转轮虫和旋轮虫的ND4和ND5基因序列,使用MEGA6.0软件构建这12种轮虫系统发生树(NJ树、ME树、UPGMA树),探讨了8种臂尾轮虫之间的系统关系。结果显示,本研究所涉及的轮虫ND4和ND5基因序列差异百分比均值为40.3%,可作为分子标记应用于轮虫属内种间系统关系研究;系统树所显示亲缘关系与形态学研究基本一致,均支持将十指臂尾轮虫、裂足臂尾轮虫归入臂尾轮属。
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| 关 键 词： | 轮虫 臂尾轮属 ND4 ND5 系统发生关系 |
| 收稿时间： | 2016/1/20 0:00:00 |
| 修稿时间： | 7/5/2016 12:00:00 AM |
Phylogenetic relationship and taxonomic status of eight Brachionus species (Rotifera) inferred from ND4 and ND5 genes |
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| CHENG Shuanghuai,WEI Shixiang,ZHANG Yi,SUN Daoqin,JIANG Jing,MU Weiping,XI Yilong and GE Yali.Phylogenetic relationship and taxonomic status of eight Brachionus species (Rotifera) inferred from ND4 and ND5 genes[J].Journal of Fisheries of China,2016,40(12):1823-1829. |
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| Authors: | CHENG Shuanghuai WEI Shixiang ZHANG Yi SUN Daoqin JIANG Jing MU Weiping XI Yilong and GE Yali |
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| Institution: | College of Life Sciences, Anhui Normal University, Wuhu 241000, China;Provincial Key Laboratory of Biotic Environment and Ecological Safety in Anhui, Wuhu 241000, China;Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Wuhu 241000, China,College of Life Sciences, Anhui Normal University, Wuhu 241000, China,College of Life Sciences, Anhui Normal University, Wuhu 241000, China,College of Life Sciences, Anhui Normal University, Wuhu 241000, China,College of Life Sciences, Anhui Normal University, Wuhu 241000, China,College of Life Sciences, Anhui Normal University, Wuhu 241000, China,College of Life Sciences, Anhui Normal University, Wuhu 241000, China;Provincial Key Laboratory of Biotic Environment and Ecological Safety in Anhui, Wuhu 241000, China;Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Wuhu 241000, China and College of Life Sciences, Anhui Normal University, Wuhu 241000, China;Provincial Key Laboratory of Biotic Environment and Ecological Safety in Anhui, Wuhu 241000, China;Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Wuhu 241000, China |
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| Abstract: | Partial portions of ND4 and ND5 gene from Brachionus caudatus, B. diversicornis, B. forficula, B. quadridentatus, B. rubens, B. falcatus, B. leydigi, B. patulus were amplified and sequenced. Combined with ND4 and ND5 gene of B. plicatilis, B. calyciflorus, Rotaria rotatoria and Philodina citrina from GenBank, phylogenetic tree of neighbor-joining (NJ), minimum-evolution (ME) and unweighted pair-group method using an arithmetic average (UPGMA) methods were built with MEGA6.0 software to reveal their phylogenetic relationships and resolve some taxonomy questions. The results showed that the average percentage of divergence for ND4 and ND5 gene sequences in 12 samples was 40.3%. ND4 and ND5 gene was suitable for researching the phylogenesis and evolutionary of rotifera species in the genus. The relationships of Brachionus showing from the phylogenetic trees were basically consistent with morphological studies. All the three trees showed that B. patulus and B. diversicornis were confirmed to belong to the genus Brachionus. |
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| Keywords: | rotifera Brachionus ND4 ND5 phylogenetic relationship |
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