肌注和口服氟苯尼考在中华鳖体内残留分析及药代动力学

研究不同给药条件下，氟苯尼考在中华鳖体内的残留及药代动力学特征。健康中华鳖160只，随机分为2组，按30 mg·kg^-1剂量分别单次肌注或口服氟苯尼考，高效液相色谱法测定中华鳖血浆和肌肉药物残留浓度，利用3P87药代动力学软件分析数据。肌注和口服时均符合一室开放模型；肌注给药的动力学方程C=16.72(e^-0.15t-e^0.52t) ，主要药代动力学参数：AUC=76.45 μg·mL^-1·h^-1，吸收半衰期（T_1/2Ka）=1.31 h，半衰期(T_1/2Ke)=4.48 h，最高血药浓度C_max=7.09 μg·L^-1；口服给药的动力学 方程为C=39.99(e^-0.19t-e^0.4t)，主要药代动力学参数：AUC=109.42 μg·mL^-1·h^-1，吸收半衰期（T_1/2Ka）=1.73 h，半衰期(T_1/2Ke)=3.63 h，最高血药浓度C_max=10.64 〗μg·L^-1。实验结果表明，氟苯尼考口服情况下，在中华鳖体内吸收快，血药浓度高，维持时间长，生物利用度高；药物在肌肉中消除缓慢。

The residues and pharmacokinetics of florphenicol in Trionyx sinensis following intramascular injection and oral administration

To study the residues and pharmacokinetics of florphenicol in Trionyx sinensis with different treatments, one hundred and sixty healthy T. sinensis were selected and followed by a single dose of florphenicol (30 mg·kg_-1) with intramascular injection and oral administration respectively. Serial serum samples were obtained and assayed by reversed phase HPLC, and the corresponding pharmacokinetic parameters were calculated with 3p87. A one compartment open model best fits the data. The major pharmacokinetic parameters were as follows: AUC=76.45 mg·L^-1·h^-1, T_1/2ka=1.31 h, T_1/2ke=4.48 h, and C_max=7.09 mg·L^-1 in intramascular injection group; while AUC =109.42 mg·L^-1·h^-1, T^1/2ka=1.73 h, T_1/2ke=3.63 h, and C_max=10.64 mg·L^-1 in oral administration one. It suggested that the assimilation of florphenicol was faster, the level of risidue concentration was higher, the maintain time was longer, and the bioavailability was higher in oral administration group as compared to intramascular injection. Florphenicol residue eliminated slowly in musculature.