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用稳定性同位素技术分析稻—虾系统中不同“碳/氮”投喂方式对克氏原螯虾食性的影响
引用本文:李聪,成永旭,管勤壮,奚业文,李嘉尧.用稳定性同位素技术分析稻—虾系统中不同“碳/氮”投喂方式对克氏原螯虾食性的影响[J].水产学报,2018,42(11):1778-1786.
作者姓名:李聪  成永旭  管勤壮  奚业文  李嘉尧
作者单位:上海海洋大学水产科学国家级实验教学示范中心;上海海洋大学水产动物遗传育种中心上海市协同创新中心;上海海洋大学农业部淡水水产种质资源重点实验室;安徽省水产技术推广总站
基金项目:上海市科委农业领域科技支撑项目(15391912100);江苏省渔业科技类项目(D2017-1-1);上海高校水产学高峰学科建设专项(2015-62-0908)
摘    要:为改善克氏原螯虾在稻—虾共作模式下对稻田中丰富自然资源的利用效率,本研究通过在饲料中添加碳源(麸皮、葡萄糖)调节饲料"碳/氮"(C/N)分别为8,12,16三个组别进行投喂,利用稳定性同位素技术分析各组克氏原螯虾的食性。结果显示,(1)不同C/N投喂组中不同规格成虾肌肉中的δ13C和δ15N值均较本底显著降低,成虾肌肉的δ13C值随投喂饲料C/N的增加而下降,B组不同规格成虾肌肉的δ15N值均较其他两组高。同一C/N投喂处理组中,1级小规格成虾肌肉的δ13C和δ15N值均为最高。(2)通过食性分析,组A中不同规格的克氏原螯虾的第一食物来源均为饲料,占比38.03%~44.17%,组B中饲料仍为不同规格克氏原螯虾的第一食物来源,但是占比下降为22.13%~25.35%,组C中1级小规格成虾中有机碎屑为第一食物来源,占19.93%,2级中规格成虾及3级大规格成虾的第一食物来源饲料进一步下降为18.85%和19.40%,组B及组C中浮游动物、藻类、喜旱莲子草等天然饵料食物来源占比均较组A显著增加。研究表明,通过优化C/N的投喂模式,可以提高克氏原螯虾对于稻田天然饵料的利用率,避免饲料的过度使用和浪费,有利于实现对稻田资源的合理利用。

关 键 词:克氏原螯虾  稻-虾共作  C/N  稳定性同位素技术  食性分析
收稿时间:2017/12/29 0:00:00
修稿时间:2018/3/27 0:00:00

Using stable isotopes to estimate the effects of different carbon/nitrogen feeds on feeding habit of Procambarus clarkii in the rice-crayfish co-culture system
LI Cong,CHENG Yongxu,GUAN Qinzhuang,XI Yewen and LI Jiayao.Using stable isotopes to estimate the effects of different carbon/nitrogen feeds on feeding habit of Procambarus clarkii in the rice-crayfish co-culture system[J].Journal of Fisheries of China,2018,42(11):1778-1786.
Authors:LI Cong  CHENG Yongxu  GUAN Qinzhuang  XI Yewen and LI Jiayao
Institution:Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China,Fisheries Technology Extension Center of Anhui Province, Hefei 230601, China and Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
Abstract:
Keywords:Procambarus clarkii  rice-crayfish co-culture  C/N  stable isotopes  diets analysis
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