抑食金球藻对翡翠贻贝抗氧化酶系统的影响

本研究以单种抑食金球藻及其与亚心形四爿藻的混合藻为实验组,以亚心形四爿藻为对照,探究抑食金球藻对翡翠贻贝超氧化物歧化酶(SOD)活性、丙二醛(MDA)和谷胱甘肽(GSH)含量的影响。结果显示,抑食金球藻显著影响翡翠贻贝的SOD活性、MDA和GSH含量,各实验组均表现出诱导升高与抑制降低交替出现的规律。单种高、中浓度的抑食金球藻在短期(3 h)内即可对翡翠贻贝的SOD活性产生影响,混合藻组中的SOD活性上升较慢,说明亚心形四爿藻可以延滞氧化还原系统的反应时间。各实验组翡翠贻贝GSH含量在2 d内均显著低于对照组,且混合藻组与单种抑食金球藻组相差不大,提示亚心形四爿藻对抗氧化还原损伤并未起到明显的缓解作用。

Effect of Aureococcus anophagefferens on the antioxidant enzyme system in Perna viridis

Effects of Aureococcus anophagefferens on superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) contents of mussel Perna viridis were studied by feeding P. viridis with pure culturing A. anophagefferens and co-occurring algae (A. anophagefferens and Tetraselmis subcordiformis). Mussels fed with T. subcordiformis were set as control. The results showed that A. anophagefferens significantly effected the antioxidant enzyme system in mussel P. viridis including SOD, MDA, and GSH. SOD activity and MDA, GSH contents fluctuated after P. viridis exposing to A. anophagefferens. The high and moderate cell densities of A. anophagefferens can affect the SOD activity significantly within a short time (3 h). SOD activity in the mussel exposing to the co-occurring algae, by contrast, increased slowly, suggesting that T. subcordiformis can prolong reaction time of the antioxidant system. Compared with control group, GSH content in each experiment group was significantly reduced in 2 d. GSH contents had no difference in the mussel exposed to co-occurring algae and pure culturing A. anophagefferens, which suggested that T. subcordiformis has no positive effect on restoration of the antioxidant system in mussels.