| 中国明对虾Dscam基因的克隆及其在免疫致敏(类免疫)诱导反应中的表达分析 |
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| 引用本文： | 曹家旺,孟宪红,孔杰,史晓丽,栾生,罗坤,董丽君,陈宝龙.中国明对虾Dscam基因的克隆及其在免疫致敏(类免疫)诱导反应中的表达分析[J].水产学报,2018,42(5):633-645. |
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| 作者姓名： | 曹家旺 孟宪红 孔杰 史晓丽 栾生 罗坤 董丽君 陈宝龙 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室;青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 |
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| 基金项目： | 中国水产科学研究院黄海水产研究所基本科研业务费专项（20603022017001）；国家自然科学基金（31372523）；泰山学者种业团队项目；国家现代农业产业技术体系（CARS-48） |
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| 摘 要： | 为了研究对虾免疫致敏过程中Dscam基因的表达规律,实验采用同源克隆和RACE技术获得了中国明对虾Dscam基因c DNA全长序列,并对该序列进行生物信息学分析。结果显示,中国明对虾Dscam基因的c DNA全长为6 624 bp,其中包括171 bp的5′端非编码区,459 bp的3′端非编码区,开放阅读框的长度为5 994 bp,编码1 996个氨基酸。推测该基因编码的蛋白含有一个信号肽、10个Ig结构域、6个FNIII功能区、1个胞质尾区和1个跨膜结构域。同源性分析及系统进化分析表明,Dscam基因与节肢动物的Dscam基因首先聚为一类,且与凡纳滨对虾的同源性最高,为92.4%。连续投喂6 d热灭活白斑综合征病毒(white spot syndrome virus,WSSV)饵料来诱导免疫致敏反应,在0、6和12 d及二次感染后的12、24、48、72和168 h分别取样,用RT-PCR的方法检测中国明对虾Dscam的相对表达量。结果显示,诱导感染组Dscam基因在第12天开始上调,且与阴性对照组和未诱导感染组差异显著;二次感染后24 h,Dscam基因的相对表达量达到最大值,与阴性对照组和未诱导感染组差异显著;48 h后基因表达量开始下降,但表达量仍高于阴性对照组和未诱导感染组。实验表明,中国明对虾存在Dscam基因,并在免疫致敏过程中发挥重要作用。
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| 关 键 词： | 中国明对虾 Dscam基因 免疫致敏 表达分析 |
| 收稿时间： | 2017/5/23 0:00:00 |
| 修稿时间： | 2017/11/4 0:00:00 |
Cloning and expression analysis of the Dscam gene during the inducing immune priming (Quasi-immune) response in Fenneropenaeus chinensis |
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| CAO Jiawang,MENG Xianhong,KONG Jie,SHI Xiaoli,LUAN Sheng,LUO Kun,DONG Lijun and CHEN Baolong.Cloning and expression analysis of the Dscam gene during the inducing immune priming (Quasi-immune) response in Fenneropenaeus chinensis[J].Journal of Fisheries of China,2018,42(5):633-645. |
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| Authors: | CAO Jiawang MENG Xianhong KONG Jie SHI Xiaoli LUAN Sheng LUO Kun DONG Lijun and CHEN Baolong |
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| Institution: | Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China,Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China and Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China |
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| Abstract: | |
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| Keywords: | Fenneropenaeus chinensis Dscam immune priming expression analysis |
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