小麦蛋白粉替代鱼粉对大黄鱼幼鱼生长、血清生化指标及抗氧化能力的影响

为研究小麦蛋白粉替代大黄鱼幼鱼饲料中的鱼粉对其生长性能、体成分、血清生化指标及肝脏抗氧化能力的影响,实验以小麦蛋白粉替代基础饲料中0%(FM组为对照组)、25%(WGM25组)、50%(WGM50组)、75%(WGM75组)和100%(WGM100组)的鱼粉,配制成5种等氮(蛋白质水平为45%)等脂(脂肪水平为10%)的饲料。结果显示,大黄鱼幼鱼各处理组的存活率(SR)和饲料系数(FCR)差异不显著;增重率(WGR)和特定生长率(SGR)各替代组显著高于对照组;各处理组的肝体比(HSI)、脏体比(VSI)和肥满度(CF)差异不显著。全鱼粗蛋白、粗脂肪、水分和灰分的含量差异不显著;肌肉粗蛋白、粗脂肪、水分含量差异不显著;肌肉灰分含量WGM50、WGM75组和FM、WGM25、WGM100组差异显著。各处理组大黄鱼血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的含量均无显著性差异。肝脏超氧化物歧化酶(SOD)的活性,WGM25和WGM100组显著低于FM、WGM50和WGM75组;丙二醛(MDA)的活性,WGM50组显著高于FM、WGM25和WGM100组;过氧化氢酶(CAT)的活性,各组间差异不显著;谷胱甘肽过氧化物酶(GPx)的活性,WGM100组显著高于其他组。研究表明,在该实验条件下,小麦蛋白粉替代饲料(含40%鱼粉)中100%的鱼粉不会影响大黄鱼幼鱼的生长。

Effect of replacing fish meal with wheat gluten meal on growth, serum biochemical indexes and antioxidant enzyme activity of juvenile large yellow croaker (Larimichthys crocea)

A 56 d feeding experiment was conducted to investigate the effects of replacement of fish meal (FM) with wheat gluten meal (WGM) in diets on the growth, body composition, serum biochemical indexes and liver antioxidant ability of juvenile large yellow croaker, Larimichthys croceainitial weight (10.49±0.03)g]. The basal diets were formulated by replacing 0% (FM), 25% (WGM25), 50% (WGM50), 75% (WGM75), 100% (WGM100) FM with WGM. The results showed that there were no significant differences in survival rate (SR), feed conversion ratio (FCR), hepatosomatic index (HSI), visceasomatic index (VSI) and condition factor (CF) among all diets; there were significant differences between the treatment and the control diets in the weight gain rate (WGR) and the specific growth rate (SGR). There were no significant differences in whole body crude protein, crude lipid, water and ash content. There were no significant differences in muscle crude protein, crude lipid and water content, but in muscle ash content, there were significant differences between WGM50, WGM75 diets and FM, WGM25, WGM100 diets. There were no significant differences in serum alanine aminotransferase (ALT) and aspartate amino-transferase (AST) content. For liver superoxide dismutase (SOD) activity, it was significantly lower in WGM25, WGM100 diets than FM, WGM50 and WGM75 diets; for liver malondialdehyde (MDA) activity, it was significantly lower in WGM50 diet than FM, WGM25 and WGM100 diets; for liver catalase (CAT) activity, there were no significant differences; for liver glutathione peroxidase (GPx) activity, it was significantly higher in WGM100 diet than the other diets. From what have been discussed above, we can conclude that replacing 100% FM with WGM is suitable for juvenile large yellow croaker, and would not affect the growth performance.