| 杜仲对草鱼生长、肌肉品质和胶原蛋白基因表达的影响 |
| |
| 引用本文： | 许晓莹,李小勤,孙文通,姜维波,潘雯倩,汪军鹏,土志涵,冷向军.杜仲对草鱼生长、肌肉品质和胶原蛋白基因表达的影响[J].水产学报,2018,42(5):787-796. |
| |
| 作者姓名： | 许晓莹 李小勤 孙文通 姜维波 潘雯倩 汪军鹏 土志涵 冷向军 |
| |
| 作者单位： | 水产科学国家级实验教学示范中心上海海洋大学;农业部鱼类营养与环境生态研究中心上海海洋大学;水产动物遗传育种中心上海市协同创新中心上海海洋大学 |
| |
| 基金项目： | 国家自然科学基金（31772858） |
| |
| 摘 要： | 为研究杜仲对草鱼生长性能、肌肉品质及胶原蛋白基因COL1A1和COL1A2表达的影响,实验采用初始体质量为(215.0±0.4)g的草鱼120尾,随机分为2处理组(每组3重复,每重复20尾鱼),分别饲喂基础饲料(对照组)和添加2%杜仲的实验饲料(杜仲组),养殖时间为8周。结果显示,与对照组相比,添加2%杜仲对草鱼生长性能无显著影响,但能显著增加肌肉、皮肤和肝脏胶原蛋白水平,增加肌肉总必需氨基酸(TEAA)、总氨基酸(TAA)水平。2%杜仲可显著降低草鱼肌肉的冷冻失水率、离心失水率,但对肌纤维密度和肌纤维直径无显著影响。在胶原蛋白基因表达方面,2%杜仲显著增加了第4周、8周时草鱼的肌肉、皮肤和第8周时的肝脏组织COL1A1、COL1A2基因m RNA表达量。研究表明,饲料中添加2%杜仲可改善大规格草鱼的肌肉品质。
|
| 关 键 词： | 草鱼 杜仲 生长 胶原蛋白 肌肉品质 COL1A1 COL1A2 基因表达 |
| 收稿时间： | 2017/4/24 0:00:00 |
| 修稿时间： | 2017/10/11 0:00:00 |
Effects of dietary Eucommia ulmoides on growth, flesh quality, and collagen gene expression of grass carp (Ctenopharyngodon idella) |
| |
| XU Xiaoying,LI Xiaoqin,SUN Wentong,JIANG Weibo,PAN Wenqian,WANG Junpeng,TU Zhihan and LENG Xiangjun.Effects of dietary Eucommia ulmoides on growth, flesh quality, and collagen gene expression of grass carp (Ctenopharyngodon idella)[J].Journal of Fisheries of China,2018,42(5):787-796. |
| |
| Authors: | XU Xiaoying LI Xiaoqin SUN Wentong JIANG Weibo PAN Wenqian WANG Junpeng TU Zhihan and LENG Xiangjun |
| |
| Institution: | National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China,National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China,National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China and National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China |
| |
| Abstract: | The present study was conducted to investigate the effects of dietary Eucommia ulmoides (EU) on growth performance, flesh quality, collagen genes COL1A1 and COL1A2 expression of grass carp (Ctenopharyngodon idella). A total of 120 grass carp with an initial body weight of (215.0±0.4) g were randomly allocated into 2 groups with 3 replicates per group and 20 fish per replicate. The fish were fed formula diet without (control) or with the supplementation of 2% Eucommia ulmoides (EU) for 8 weeks. The supplementation of 2% EU did not significantly affect the growth performance of grass carp when compared with the control (P>0.05), but increased the collagen content in muscle, skin and liver (P<0.05). Total essential amino acids (TEAA), total non-essential amino acids (TNEAA) and total amino acids (TAA) in muscle of grass carp fed EU diet were significantly higher than those of control group. Fish fed EU showed significantly lower flesh thawing loss and centrifugal loss (higher water holding capacity) (P<0.05), but muscle fiber density and muscle fiber diameter showed no difference between the two groups (P>0.05). Moreover, dietary EU significantly promoted the relative expression level of COL1A1 and COL1A2 mRNA in muscle, skin at the 4th week, the 8th week, and the expression in liver at the 8th week (P<0.05). In conclusion, dietary EU could improve the flesh quality of large-size grass carp. |
| |
| Keywords: | Ctenopharyngodon idella Eucommia ulmoides growth collagen flesh quality COL1A1 COL1A2 gene expression |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
