大菱鲆饲料转化率相关微卫星标记的筛选

饲料转化率(FCR)是大菱鲆重要的经济性状，通过选择育种提高饲料转化率，能够有效地降低大菱鲆的养殖成本，进而推动产业的发展。微卫星标记是鱼类分子标记辅助选育中常用的分子标记，为了筛选出与大菱鲆饲料转化率相关的微卫星标记，提高育种效率，实验以300尾大菱鲆幼鱼为研究对象，通过特制的网箱养殖系统，测定个体饲料转化率，分别选取饲料转化率最高和最低的30个样本作为高饲料转化率组(H组)和低饲料转化率组(L组)。利用40对大菱鲆微卫星引物，对H组和L组的DNA混池进行PCR扩增，统计两组个体PCR产物的基因型，筛选两池之间出现差异等位基因片段的位点，通过进一步的群体验证和家系验证，分析微卫星位点与大菱鲆饲料转化率的相关性。结果显示，微卫星位点YSKr148在238 bp的等位基因片段与大菱鲆饲料转化率存在极显著正相关，相关系数达到0.359，家系验证中该位点的阳性组的饲料转化率显著高于阴性组。研究表明，大菱鲆微卫星位点YSKr148与饲料转化率性状显著相关，可以用于该性状的分子标记辅助选育。本研究首次获得了与大菱鲆饲料转化率性状显著相关的分子标记，为研究该性状的遗传基础以及相关分子机制提供了依据...

Screening of microsatellite markers associated with feed conversion ratio in Scophthalmus maximus

Feed conversion ratio (FCR, %) is an important economic trait of turbot (Scophthalmus maximus). Improving feed conversion ratio through selective breeding can effectively reduce the breeding cost and improve the breeding profit, thus promoting the development of the industry. Microsatellite markers are commonly used in molecular marker-assisted breeding of fish. In order to screen out microsatellite markers related to feed conversion ratio of turbot and improve breeding efficiency, 300 juvenile turbot were used as experimental materials in this study, and individual feed conversion ratio was measured through a special cage culture system. The 30 samples with the highest and lowest feed conversion ratio were selected as the high feed conversion ratio group (group H) and the low feed conversion ratio group (group L). 40 pairs of microsatellite primers were used to amplify the DNA mixed pools of turbot from groups H and L by PCR. The genotypes of individual PCR products of the two groups were counted, and the loci with different allele fragments between the two pools were screened. Through further population verification and family verification, the correlation between microsatellite loci and feed conversion ratio of turbot was analyzed. The results showed that the allele of microsatellite locus YSKr148 at 238 bp was significantly positively correlated with the feed conversion ratio of turbot (P < 0.01), and the correlation coefficient reached 0.359. The feed conversion ratio of the positive group was significantly higher than that of the negative group in family verification (P < 0.01). In this study, for the first time, molecular markers significantly related to feed conversion ratio of turbot were screened, which provided a basis for studying the genetic basis and related molecular mechanism of this trait, and laid a foundation for molecular marker-assisted breeding of this trait.