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| 鲤头长、体厚、体高性状的QTL定位及遗传效应分析 | |
| 引用本文： | 王宣朋,李文升,孙效文,张天奇,张晓峰.鲤头长、体厚、体高性状的QTL定位及遗传效应分析[J].水产学报,2010,34(11):1645-1655. |
| 作者姓名： | 王宣朋 李文升 孙效文 张天奇 张晓峰 |
| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;上海海洋大学水产与生命学院,上海,201306 2. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070 3. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;大连海洋大学生命科学与技术学院,大连,116023 |
| 基金项目： | 水产分子育种共性技术的建立与应用(200903045) |
| 摘 要： | 用174个SSR、41个EST、345个SNP标记对以镜鲤良种后代为祖父母本所培育的杂交F2群体的68个个体进行基因型检测,运用JoinMap4.0软件包构建遗传连锁图。利用MapQTL5.0区间作图法(interval mapping,IM)和多QTL区间定位法(MQM mapping,MQM)进行QTL检测,通过置换实验(1000次重复)确定连锁群显著性水平阈值。在对体高、头长、体厚的区间定位中,共检测到6个与体高性状相关的QTLs区间,分布在LG1(SNP1339-SNP1490)、LG10(HLJE469-SNP1491)、LG12(SNP0922-HLJ1316)、LG13(SNP0937-HLJ328)、LG25(SNP1041-HLJ594)、LG35(SNP1425-SNP0389)等6个连锁群上,解释表型变异范围为20.0%～43.3%。其中,SNP1339-SNP1490区间LOD值最大为3.64,解释表型变异35.4%。6个与头长相关的QTLs,分布在LG1(SNP1339-SNP1490)、LG12(HLJ071-HLJ336)、LG13(SNP0937-HLJ328)、LG24(SN... |
| 关 键 词： | 鲤 头长 体高 体厚 数量性状定位 |
| 收稿时间： | 2010/7/16 0:00:00 |
| 修稿时间： | 2010/8/26 0:00:00 |
Mapping and genetic effect analysis of quantitative trait loci related to head length,body height,and body thickness of common carp(Cyprinus carpio L.) |
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| WANG Xuan-peng,LI Wen-sheng,SUN Xiao-wen,ZHANG Tian-qi and ZHANG Xiao-feng.Mapping and genetic effect analysis of quantitative trait loci related to head length,body height,and body thickness of common carp(Cyprinus carpio L.)[J].Journal of Fisheries of China,2010,34(11):1645-1655. | |
| Authors: | WANG Xuan-peng LI Wen-sheng SUN Xiao-wen ZHANG Tian-qi and ZHANG Xiao-feng |
| Institution: | WANG Xuan-peng1,2,ZHANG Xiao-feng1,ZHANG Tian-qi1,3,LI Wen-sheng1,SUN Xiao-wen1 (1.Heilongjiang Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,3.College of Aqua-life Science and Technology,Dalian Ocean University,Dalian 116023,China) |
| Abstract: | A group of F2 hybrids common carp including 68 individuals was used to construct a linkage map using 560 markers(174 SSR markers,41EST-SSR markers and 345 SNP markers) .Quantitative traits loci (QTLs) associated with body hieght,head length,body thickness were identified by interval mapping and MQM Mapping of the software MapQTL5.0.A linkage group wide permutation test(1 000 replicates) determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group.Six QTLs w... |
| Keywords: | common carp (Cyprinus carpio) head length body height body thickness quantitative trait locus |
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