| 坛紫菜优良品系的选育与特性分析 |
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| 引用本文： | 蒋悦,严兴洪,刘长军.坛紫菜优良品系的选育与特性分析[J].水产学报,2010,34(9):1363-1370. |
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| 作者姓名： | 蒋悦 严兴洪 刘长军 |
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| 作者单位： | 1. 上海海洋大学水产与生命学院,上海,201306
2. 浙江省象山县水产养殖技术推广站,浙江,象山,315700 |
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| 基金项目： | 国家自然科学基金项目(30571443); 国家“八六三”高技术研究发展计划(2006AA10A413); 上海市优秀学科带头人项目(07XD14028); 上海市教委重点学科建设项目(J50701); 农业部公益性专项(200903030-C); 宁波市重大科技攻关委托项目(200702C1011026) |
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| 摘 要： | 利用体细胞克隆技术,从象山湾采集回来的坛紫菜叶状体的体细胞再生群体中筛选出细长型生长快的叶状体,再次进行体细胞克隆,从它的体细胞再生体中再次选育出生长最快的叶状体,依此法连续筛选3次,最后获得一个快速生长的优良品系(XS-1),其F1代的叶状体在生长速度、成熟期、3种主要光合色素含量等方面明显优于野生型品系(wt)。在相同条件下培养80d,XS-1品系的F1代叶状体平均体长达128.8cm,是wt品系的10.45倍;XS-1品系的F1叶状体群体的成熟高峰出现时间比wt品系推迟20d;在波长350～750nm之间,两个品系的叶状体活体吸收光谱中均出现5个吸收峰,但XS-1品系的各峰峰值均远高于wt品系;XS-1品系的叶状体总藻胆蛋白含量高达80.4mg/g,比wt品系提高了188%,而它的Chl.a含量比wt品系提高了32%;XS-1品系的叶状体平均厚度为32.2μm,比wt品系减少15%。研究结果表明,XS-1品系是一个生长快、颜色和品质好、遗传稳定的优良品系,有望在生产中得到应用。
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| 关 键 词： | 坛紫菜 优良品系 生长率 活体吸收光谱 藻胆蛋白 |
| 收稿时间： | 2010/4/19 0:00:00 |
| 修稿时间： | 2010/5/13 0:00:00 |
Selection and characterization of a fast growing strain of Porphyra haitanensis (Bangiales, Rhodophyta) |
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| YAN Xinghong and LIU Changjun.Selection and characterization of a fast growing strain of Porphyra haitanensis (Bangiales, Rhodophyta)[J].Journal of Fisheries of China,2010,34(9):1363-1370. |
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| Authors: | YAN Xinghong and LIU Changjun |
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| Institution: | JIANG Yue1,YAN Xing-hong1,LIU Chang-jun2 ( 1. College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China,2. Xiangshan Fisheries Technical Extension Center,Xiangshan 315700,China) |
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| Abstract: | From the regenerated blades of single somatic cells isolated enzymatically from a blade collected from the cultivation ground of Porphyra haitanensis Chang et Zheng,the fastest growing blade was selected for isolating somatic cells,and was cultured for getting regeneration blades again. From its regenerated blades,the fastest growing blade was isolated. After 3 times of selections continuously in this way,we got an improved strain named XS-1,characterized by faster growing,later maturation and higher conten... |
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| Keywords: | Porphyra haitanensis improved strain growth rate in vivo absorption spectra phycobiliprotein |
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