| 中华鳖MyD88部分序列克隆及其在组织中的表达差异分析 |
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| 引用本文： | 朱炳林,李俊,方维焕,李肖梁.中华鳖MyD88部分序列克隆及其在组织中的表达差异分析[J].水产学报,2010,34(7):1018-1024. |
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| 作者姓名： | 朱炳林 李俊 方维焕 李肖梁 |
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| 作者单位： | 1. 浙江大学动物科学学院,浙江省动物预防医学重点实验室,浙江,杭州,310029
2. 西南大学动物科技学院,重庆,400716 |
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| 基金项目： | 国家“十一五”科技支撑计划(2009BADB9B09-5); 杭州市重大专项资助项目(20082332Y03) |
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| 摘 要： | MyD88(Myeloid differentiation factor 88,MyD88)是多数TLRs(Toll like receptor,TLRs)信号转导过程中的关键接头分子。研究根据MyD88(Myeloid differentiation factor 88,MyD88)TIR结构域(Toll-like/IL-1 receptor domain)保守区设计简并引物,通过PCR扩增,成功地从中华鳖脾脏cDNA中扩增出351bp的目标序列。测序后经结构域和序列比较分析,扩增片段为中华鳖的MyD88 TIR结构域。该序列与大黄鱼、鸡等脊椎动物的MyD88的TIR结构域序列同源性和相似性分别为72.9%～86%和77.6%～83.6%。以热灭活嗜水气单胞菌刺激中华鳖后,经荧光定量PCR检测,在48h内,肝、脾和肾组织中MyD88 mRNA相对表达量均出现不同程度的增加,尤以脾脏中的增幅最为明显,为对照组的6.89倍;中华鳖心脏成纤维样细胞经20ng LPS刺激后1～8h,MyD88表达量有所提高,24h的表达量最高。该研究结果将为开展中华鳖天然免疫奠定良好基础。
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| 关 键 词： | 中华鳖 髓样分化因子88 TIR结构域 组织表达 嗜水气单胞菌 脂多糖 |
| 收稿时间： | 4/8/2010 12:00:00 AM |
| 修稿时间： | 2010/5/17 0:00:00 |
Cloning of MyD88 partial cDNA sequence of Chinese soft shelled turtle (Trionyx sinensis) and its expression in different tissues |
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| zhubinglin,LI Jun,FANG Weihuan and LI Xiaoliang.Cloning of MyD88 partial cDNA sequence of Chinese soft shelled turtle (Trionyx sinensis) and its expression in different tissues[J].Journal of Fisheries of China,2010,34(7):1018-1024. |
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| Authors: | zhubinglin LI Jun FANG Weihuan and LI Xiaoliang |
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| Institution: | ZHU Bing-lin1,LI Jun2,FANG Wei-huan1,LI Xiao-liang1(1.Zhejiang Province Key Lab of Preventive Veterinary Medicine,College of Animal Sciences,Zhejiang University,Hangzhou 310029,China,2.College of Animal Science and Technology,Southwest University,Chongqing 400716,China) |
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| Abstract: | Myeloid differentiation factor 88(MyD88),which is considered as a pivot adaptor molecule for signal transduction of all toll-like receptors(TLRs) except TLR3,possesses important function.In this study,degenerate primer pairs were designed from the conserved regions of the Toll-like/IL-1 receptor(TIR) domain of myeloid differentiation factor 88(MyD88).A 351-bp length target sequence was amplified by PCR successfully from the spleen cDNA of Chinese soft-shelled turtle(CSST),and is found as the TIR domain of C... |
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| Keywords: | Trionyx sinensis(Chinese soft-shelled turtle) myeloid differentiation factor 88(MyD88) toll-like/IL-1 receptor(TIR)domain tissue expression Aeromonas hydrophila lipopolysaccharides(LPS) |
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