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| 条斑紫菜HSP90基因的克隆与表达分析 | |
| 引用本文： | 周向红,李信书,王萍,阎斌伦,滕亚娟,易乐飞.条斑紫菜HSP90基因的克隆与表达分析[J].水产学报,2010,34(12):1844-1852. |
| 作者姓名： | 周向红 李信书 王萍 阎斌伦 滕亚娟 易乐飞 |
| 作者单位： | 1. 淮海工学院江苏省海洋生物技术重点实验室,江苏,连云港,222005;淮海工学院海洋学院,江苏,连云港,222005 2. 淮海工学院海洋学院,江苏,连云港,222005 3. 淮海工学院江苏省海洋生物技术重点实验室,江苏,连云港,222005 |
| 基金项目： | 国家“十一五”科技支撑计划重大项目(2006BAD09A01); 江苏省海洋生物技术重点实验室开放课题(2008HS004) |
| 摘 要： | 为了研究HSP90在条斑紫菜胁迫耐受中的作用,克隆了条斑紫菜HSP90基因(命名为PyHSP90),采用定量RT-PCR研究了其表达规律。PyHSP90基因包含一个长2274nt的完整开放阅读框,编码757个氨基酸。PyHSP90蛋白定位于细胞质中,具有HSP90蛋白家族的特征序列和保守结构域,与多种生物的HSP90具有较高的序列一致性。在进化树上条斑紫菜等红藻的HSP90与隐藻的亲缘关系最近,与绿藻和陆生植物亲缘关系较远。低温和高温胁迫都能显著性地诱导条斑紫菜叶状体PyHSP90基因的表达,而且表达量与胁迫程度成正相关;低盐胁迫下PyHSP90基因表达上调;而失水胁迫下PyHSP90基因表达下调。 |
| 关 键 词： | 条斑紫菜 热应激蛋白90 克隆 表达 定量PCR |
| 收稿时间： | 2010/7/11 0:00:00 |
| 修稿时间： | 2010/9/24 0:00:00 |
Molecular cloning and expression analysis of HSP90 gene from Porphyra yezoensis Ueda(Bangiales, Rhodophyta) |
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| ZHOU Xiang-hong,LI Xin-shu,WANG Ping,YAN Bin-lun,TENG Ya-juan and YI Le-fei.Molecular cloning and expression analysis of HSP90 gene from Porphyra yezoensis Ueda(Bangiales, Rhodophyta)[J].Journal of Fisheries of China,2010,34(12):1844-1852. | |
| Authors: | ZHOU Xiang-hong LI Xin-shu WANG Ping YAN Bin-lun TENG Ya-juan and YI Le-fei |
| Institution: | ZHOU Xiang-hong1,2,LI Xin-shu1,WANG Ping2,YAN Bin-lun1,TENG Ya-juan2,YI Le-fei1,2(1. Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China,2. School of Marine Science & Technology,China) |
| Abstract: | The 90-ku heat shock proteins(HSP90s) are a group of evolutionarily highly conservative molecular chaperones and essential for viability in eukaryotes. The basic functions of HSP90s are of assisting protein folding,protein degradation and protein trafficking,and they also play an important role in signal transduction networks,cell cycle control and morphological evolution. Although HSP90s are constitutively expressed in most organisms,their expression is up-regulated in response to stresses such as cold,hea... |
| Keywords: | Porphyra yezoensis Ueda heat shock protein 90 (HSP90) cloning expression real-time PCR |
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