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山莨菪碱提高嗜水气单胞菌灭活疫苗浸泡免疫鲫的效果
引用本文:时云朵,任燕,张德锋,付小哲,林强,李宁求,石存斌,吴淑勤.山莨菪碱提高嗜水气单胞菌灭活疫苗浸泡免疫鲫的效果[J].水产学报,2015,39(5):720-727.
作者姓名:时云朵  任燕  张德锋  付小哲  林强  李宁求  石存斌  吴淑勤
作者单位:1. 中国水产科学研究院珠江水产研究所,广东省水产动物免疫技术重点实验室,农业部渔药创制重点实验室,广东广州510380;上海海洋大学水产与生命学院,上海201306
2. 中国水产科学研究院珠江水产研究所,广东省水产动物免疫技术重点实验室,农业部渔药创制重点实验室,广东广州510380
基金项目:"十二五"国家科技支撑计划(2012BAD25B02);现代农业产业技术体系建设专项(CARS-46)
摘    要:为研究山莨菪碱作为佐剂在细菌灭活疫苗浸泡免疫中的作用,将山莨菪碱和嗜水气单胞菌全菌灭活疫苗联合浸泡免疫异育银鲫,首次浸泡免疫7d后加强免疫1次.通过实时荧光定量PCR检测第2、4、7、11、14和21天脾脏中IgM、IL-1β、C3、C-凝集素以及溶菌酶的mRNA表达量,并在第21天进行同源菌株活菌攻击实验.结果显示,山莨菪碱组第4天IgM和IL-1 β的表达量达到最高值,分别为28.3和332.7;而无佐剂疫苗组第11天IgM和IL-1β达到最高值,分别为56.1和791.8.山莨菪碱组的补体C3、C-凝集素以及溶菌酶的表达量都显著高于无佐剂组和对照组,且表达持续时间长.活菌攻击实验表明山莨菪碱组的相对免疫保护率可达80.0%,显著高于无佐剂组的56.0%.结果表明,山莨菪碱与嗜水气单胞菌灭活疫苗共同浸泡免疫银鲫,可以增强脾脏中IgM、IL-1β、C3、C-凝集素以及溶菌酶基因表达,提高银鲫相对免疫保护率.

关 键 词:异育银鲫  山莨菪碱  灭活疫苗  浸泡免疫  免疫效果
收稿时间:2014/10/17 0:00:00
修稿时间:2014/12/3 0:00:00

Anisodamine increases efficacy of Aeromonas hydrophila inactivated vaccine in gibel carp Carassius auratus gibelio by immersion immunization
SHI Yunduo,REN Yan,ZHANG Defeng,FU Xiaozhe,LIN Qiang,LI Ningqiu,SHI Cunbin and WU Shuqin.Anisodamine increases efficacy of Aeromonas hydrophila inactivated vaccine in gibel carp Carassius auratus gibelio by immersion immunization[J].Journal of Fisheries of China,2015,39(5):720-727.
Authors:SHI Yunduo  REN Yan  ZHANG Defeng  FU Xiaozhe  LIN Qiang  LI Ningqiu  SHI Cunbin and WU Shuqin
Institution:Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China;College of Fishery and Life Science, Shanghai Ocean University, Shanghai 201306, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China and Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab of Aquatic Animal Immune Technology of Guangdong Province, Key Lab of Fishery Drug Development of Ministry of Agriculture, Guangzhou 510380, China
Abstract:
Keywords:Carassius auratus gibelio  anisodamine  inactivated vaccine  immersion immunization  immune efficacy
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