| 葡萄球菌A蛋白协同凝集试验快速检测草鱼出血病病毒 |
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| 引用本文： | 杨广智,罗毅志,叶雪平.葡萄球菌A蛋白协同凝集试验快速检测草鱼出血病病毒[J].水产学报,1991,15(1):27-33. |
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| 作者姓名： | 杨广智 罗毅志 叶雪平 |
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| 作者单位： | 浙江省淡水水产研究所 湖州
(杨广智，罗毅志)，浙江省淡水水产研究所 湖州(叶雪平) |
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| 摘 要： | 本文报道葡萄球菌 A蛋白协同凝集(SPA COA)快速检测草鱼出血病病毒的方法以及对提纯病毒、染毒细胞内病毒和病鱼组织检测的初步结果。并对该方法进行了特异性和敏感性考核以及凝集物的电镜观察。该方法快速、特异、设备简单,适合基层单位检测草鱼出血病病毒,并有可能作为草鱼出血病疫苗质量鉴定的指标之一。
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| 关 键 词： | 草鱼出血病 葡萄球菌 A蛋白 SPACOA |
| 收稿时间： | 1/1/1990 12:00:00 AM |
| 修稿时间： | 9/1/1990 12:00:00 AM |
RAPID SEROLOGICAL DIAGNOSIS OF GRASS CARP HAEMORRHAGIC VIRUS BY COAGGLUTINATION TEST USING ANTIBODY-SENSITIZED STAPHYLOCOCCI PROTEIN A |
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| Yang Guangzhi,Luo Yizhi and Ye Xueping.RAPID SEROLOGICAL DIAGNOSIS OF GRASS CARP HAEMORRHAGIC VIRUS BY COAGGLUTINATION TEST USING ANTIBODY-SENSITIZED STAPHYLOCOCCI PROTEIN A[J].Journal of Fisheries of China,1991,15(1):27-33. |
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| Authors: | Yang Guangzhi Luo Yizhi and Ye Xueping |
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| Institution: | Zhejiang Institute of Freshwater Fisheries, Huzhou;Zhejiang Institute of Freshwater Fisheries, Huzhou;Zhejiang Institute of Freshwater Fisheries, Huzhou |
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| Abstract: | This study deals with the rapid diagnosis of grass carp (Ctenopharyngodon idellus) haemorrhagic virus by coagglutination test using antibody-sensi-tized staphylococci protein A (SPA COA), as well as the preliminary experimentaldata for the diagnosis of purified vius, cell-infected virus and tissues of infectedfish. The results obtained from the specific and sensitive detection and the electro-nic microscopic observation of the coagula, indicate that this rapid and simple me-thod, which requires no special apparatus and easy to perform in the basic unit, isprobably one of the valuable indexes for identifying the vaccine quality of grasscarp haemorrhage. |
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| Keywords: | staphylococci protein A coagglutination haemorrhage of grass carp |
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