不同类群九孔鲍免疫防御机能的比较

对不同类群的九孔鲍细胞与体液免疫机能进行比较,结果表明,不同类群的九孔鲍免疫机能具有显著的差异性,复壮鲍(养殖10个月,个体大小为51.8～59.2mm)细胞与体液免疫机能都明显高于退化鲍(养殖20个月,个体大小为37.4～43.1mm)(P<0.01),与野生九孔鲍(个体大小为67.7～76.7mm)相比,复壮鲍细胞免疫机能略高于野生鲍,体液免疫机能则明显低于野生鲍。九孔鲍血细胞在吞噬活动中具有呼吸爆发产生活性氧的功能与特点,活性氧的产生与九孔鲍自身生理、环境温度以及异物数量等因素有显著的相关性(P<0.01)。环境温度由25℃下降至18℃,九孔鲍的细胞与体液免疫机能都有明显的下降。退化鲍在25℃和18℃条件下血淋巴液的抗菌活力水平差异性不明显。酚氧化酶存在于九孔鲍血淋巴液中,但其活性水平不高,在各类九孔鲍之间的差异性也不明显,不能确认为九孔鲍的主要体液免疫因子。

Comparison of the immune defense functions in different farming groups of Haliotis diversicolor supertexta

In this article, the immune functions were discussed in three different groups of the abalone, Haliotis diversicolor supertexta, and the results showed that they had a clear difference both in cellular immunity and humoral immunity functions. The degrees of cellular immunity funct ions and humoral immunity funct ions of the rejuvenative abalones were higher obviously than that of the degenerative abalones. Comparing with the wild group of abalones, the rejuvenative abalones had some higher degrees in cellular immunity functions, and obviously lower degrees in humoral immunity funct ions. With phagocytosis the hemocytes of the abalones had a respiratory burst and then turned out reactive oxygen intermediate species ( ROIs) . The ROIs were much in relation to the physical state of the abalones themselves and the environmental temperatures and the quantities of the stimulant s. Both cellular immunity functions and humoral immunity functions had a signif icant decrease as the environmental temperature drop from 25bC to 18bC, while the ant ibacterial activities of the degenerative abalones only had a little difference under the two kinds of temperatures. There was some Phenoloxidase in the hemolymph of the abalone, but their activities were low and had little difference within different groups. It could not be conf irmed as a main humoral immune factor of the abalone.