首页 | 本学科首页   官方微博 | 高级检索  
     检索      

用RAPD分析对罗非鱼遗传变异的研究及其对杂种优势的应用
引用本文:夏德全.用RAPD分析对罗非鱼遗传变异的研究及其对杂种优势的应用[J].水产学报,1999,23(1):27-32.
作者姓名:夏德全
作者单位:中国水产科学研究院淡水渔业研究中心
基金项目:国家自然科学基金,农业部科学基金
摘    要:应用RAPD技术检测了一个奥利亚罗非鱼养殖群体和湘湖、美国和沙市三个尼罗非鱼和尼罗罗非鱼在群体内或群体间存在遗传差异。OPZ06、P)Z10、OPZ12和OPZ19四个引物都有一个扩增片段具有种的特异性,可以作为临别尼罗罗非鱼和奥利亚罗非鱼的遗传标记。群体内遗传相似性指数(S)表明,湘湖、美国和沙市三群体尼罗罗非鱼都保留了较高水平的遗传变异(S分别为0.798、0.795和0824),而奥利亚罗非

关 键 词:RAPD分析  罗非鱼  遗传变异  杂种优势
收稿时间:2014/4/18 0:00:00
修稿时间:2014/4/18 0:00:00

A STUDY ON GENETIC VARIATION OF TILAPIAS FISH WITH RAPD ANALYSIS AND ITS APPLICATION TO HETEROSIS
XIA DeQuan.A STUDY ON GENETIC VARIATION OF TILAPIAS FISH WITH RAPD ANALYSIS AND ITS APPLICATION TO HETEROSIS[J].Journal of Fisheries of China,1999,23(1):27-32.
Authors:XIA DeQuan
Institution:Freshwater Fisheries Research Center, CAFS, Wuxi214081
Abstract:Three cultured populations (Xianghu, American and Shashi) of Oreochromis nilloticus and one cultured population of O.aureus were examined using RAPD method.12 primers were screened out of 20 random primers,and each of the 12 primers revealed interor/and intrapopulation genetic variation.There were four speciesspecific amplification bands (sizing 900, 1500, 1700 and 730 bp respectively) of primers OPZ06, OPZ10, OPZ12 and OPZ19, each of which could be used as molecular genetic markers to identify O.niloticusTBZ and O.aureus . Genetic similarity indices (S) suggested that the three populations of O.niloticus maintained the higher degrees of the intrapopulation genetic variation (S for the three populations of Xianghu, American and Shashi were 0.798, 0.795 and 0.824 respectively), and O.aureus had the smallest degree of genetic variation(S=0.950) because of two times of genetic bottleneck effect.The data suggest that there is the genetic potential of developing new O.niloticus varieties, while there is relatively small genetic potential in O.aureus . Interspecific genetic distances between O.aureus and three populations of O.niloticus were 0.285, 0.262 and 0.344, respectively, which suggest that the offspring of O.aureus and Shashi population of WTBXO.niloticus will develop stronger heterosis than that of other two cross combinations.
Keywords:RAPD    Oreochromis aureus        Oreochromis niloticus    Genetic variation  Heterosis  
本文献已被 CNKI 维普 等数据库收录!
点击此处可从《水产学报》浏览原始摘要信息
点击此处可从《水产学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号