首页 | 本学科首页   官方微博 | 高级检索  
     检索      

健康和患病凡纳滨对虾幼虾消化道菌群结构的比较
引用本文:杨坤杰,王欣,熊金波,裘钱玲琳,黄雷,张化俊,郭安南,李来国,张德民.健康和患病凡纳滨对虾幼虾消化道菌群结构的比较[J].水产学报,2016,40(11):1765-1773.
作者姓名:杨坤杰  王欣  熊金波  裘钱玲琳  黄雷  张化俊  郭安南  李来国  张德民
作者单位:1. 宁波大学应用海洋生物技术教育部重点实验室,浙江宁波,315211;2. 宁波大学医学院,浙江宁波,315211;3. 宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211;浙江海洋高效健康养殖协同创新中心,浙江宁波315211;4. 宁波市鄞州瞻岐椿霖养殖场,浙江宁波,315145
基金项目:国家“八六三”高技术研究发展计划(2012AA092001);国家海洋局海洋资源生物种质资源库建设项目(12PYY001SF08-NBDX-1);浙江省应用公益性项目(2016C32063);浙江省重中之重学科开放基金(XKZSC1421)
摘    要:凡纳滨对虾养殖过程中,早期阶段是病害易感阶段,而消化道菌群结构与对虾健康关系密切.因此,探讨幼虾的消化道菌群尤其是弧菌类细菌与对虾发病的关系对病害有效防治具有重要意义.本实验采用Illumina测序研究了凡纳滨对虾健康和患病幼虾的消化道细菌群落结构,并基于纯培养和16S rDNA,recA和pyrH基因序列比对,分析了幼虾消化道中弧菌的主要种类组成.结果发现,健康幼虾消化道中洳变形菌纲和厚壁菌门丰度较高,而患病幼虾中γ-变形菌纲、拟杆菌门、放线菌门和β-变形菌纲较高,其中放线菌门丰度差异显著.基于科水平的响应比分析,发现患病幼虾消化道中动性球菌科和噬菌弧菌科的丰度显著降低,而弧菌科的丰度显著升高.相似度分析发现,驱动群落变异的OTU主要来源于弧菌属、海洋杆状菌属、冷杆菌属、假交替单胞菌属以及未分类至属的红杆菌科和微杆菌科.健康幼虾消化道弧菌组成以锡那罗亚州弧菌为主,而患病幼虾消化道弧菌组成以坎氏弧菌为主.尽管健康和患病幼虾消化道内细菌群落结构整体差异不显著,但一些重要类群丰度变化显著,其特征与已知的生态功能一致.

关 键 词:凡纳滨对虾  消化道菌群  Illumina测序  弧菌  病害
收稿时间:2015/10/14 0:00:00
修稿时间:8/8/2016 12:00:00 AM

Comparison of the bacterial community structures between healthy and diseased juvenile shrimp (Litopenaeus vannamei) digestive tract
YANG Kunjie,WANG Xin,XIONG Jinbo,QIU Qianlinglin,HUANG Lei,ZHANG Huajun,GUO Annan,LI Laiguo and ZHANG Demin.Comparison of the bacterial community structures between healthy and diseased juvenile shrimp (Litopenaeus vannamei) digestive tract[J].Journal of Fisheries of China,2016,40(11):1765-1773.
Authors:YANG Kunjie  WANG Xin  XIONG Jinbo  QIU Qianlinglin  HUANG Lei  ZHANG Huajun  GUO Annan  LI Laiguo and ZHANG Demin
Institution:Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China,Medical School, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China,Chunlin Aquacultre Company, Ningbo 315145, China and Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China
Abstract:The early stage of shrimp aquaculture is susceptible to bacterial infection and the bacterial community structure in digestive tract is highly associated with shrimp health. Therefore, it is of great significance to explore the relation between shrimp bacterial community of digestive tract and juvenile shrimp disease. We investigated the intestinal bacterial community composition of healthy and diseased juvenile shrimp (Litopenaeus vannamei) by using Illumina sequencing, and explored Vibrio diverstiy based on 16S rDNA, recA and pyrH gene sequences of Vibrio isolates. The results showed that the average relative abundances of Alphaproteobacteria and Firmicutes are higher in healthy juvenile shrimp digestive tract than those in diseased one, while these of Gammaproteobacteria, Bacteridetes, Actinobacteria and Betaproteobacteria exhibited an opposite pattern and the variation in Actinobacteria is significant. In addition, response ratio analysis at the bacterial family level shows that the relative abundances of Planococcaceae and Bacteriovoracaceae families significantly decreased, while that of Vibrionaceae remarkably increased in diseased juvenile shrimp digestive tract in relation to healthy one. Similarity percentage (SIMPER) analysis revealed that the main contribution to the variation of intestinal bacterial community structure between healthy and diseased shrimp is attributed to 8 OTUs affiliated with Vibrio, unclassified Rhodobacteraceae, Marivita, Cryobacterium, unclassified Microbacteriaceae and Pseudoalteromonas. As to Vibrio assamblege composition, we found that culturable V. sinaloensis strains were the dominant species in healthy juvenile shrimp digestive tract, while V. campbellii is dominant in diseased juvenile shrimp.
Keywords:Litopenaeus vannamei  digestive tract bacterial community  Illumina sequencing  Vibrio  disease
本文献已被 万方数据 等数据库收录!
点击此处可从《水产学报》浏览原始摘要信息
点击此处可从《水产学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号