乌鳢抽出物成分的研究

分析了乌鳢(Channaargus)即杀后背肉、腹肉、尾部肉、肝脏、生殖腺中ATP关联化合物、游离氨基酸、多胺、糖元及糖酵解中间代谢物、有机酸等的含量。ATP关联化合物在肌肉中的总量为7.5～8.0μmol·g-1。ATP在背肉含量为3.9μmol·g-1,腹肉为4.1μmol·g-1,尾部肉为4.7μmol·g-1。运动激烈的尾部肉ATP占63%,含量相当高。肝脏中有少量的腺苷与肌苷酸一起被检出,据此可认为ATP的分解存在两个途径。游离氨基酸总量在背肉中为436.0mg·(100g)-1,腹肉中为405.0mg·(100g)-1,尾部肉中为356.3mg·(100g)-1。牛磺酸和甘氨酸为主要氨基酸,占68%～73%。丙氨酸和谷氨酸也相当高的含量检出。肌肉中的多胺检出为精胺和亚精胺,肝脏和生殖腺中有较高浓度的腐胺及亚精胺和精胺检出。即杀后糖元的量约占肌肉的0.5%,还有相当多的葡萄糖和6磷酸葡萄糖的糖酵解中间代谢物以及其最终产物乳酸的大量检出。

Studies on extractive components of Channa argus

In this study, ATP and its related compounds, free amino acids, polyamines and monoamines, glycogen and glycolytic intermediates and organic acid were analyzed in the dorsal, ventral and caudal muscles of snakehead Channa argus together with the liver and gonad immediately after death. Total levels of ATP and its related compounds in the muscle ranged from 7.5 μmol·g-1 to 8.0 μmol·g-1. ATP concentrations were 3.9,4.1 and 4.7 μmol·g-1 in the dorsal, ventral and caudal muscles, respectively. ATP level was high (63%) in the caudal muscle which moves hard. Small amounts of adenosine was detected in the liver together with inosinic acid. It can be considered that there exist two metabolic pathways of ATP degradation in the liver of snakehead. The total amounts of free amino acids were 436.0,405.0 and 356.3 mg·(100 g)-1 in the dorsal, ventral and caudal muscles. Taurine and glycine were major free amino acids and accounted for 68% to 73%. Alanine and glutamic acids were detected in fairly large amounts. Spermine and spermidine were detected in fairly high amounts in the muscle. In the liver and gonad putrescine was detected in the highest amounts, followed by spermidine and spermine. Immediately after death glycogen level was about 0.5% and fairly large amounts of glucose and glucose-6-phosphate, and large amounts of lactic acid were detected.