3种淡水对虾在冻藏过程中蛋白质特性的变化

以目前市场上主要的3种养殖对虾为对象,以肌原纤维蛋白盐溶解度、Ca2+-ATPase活性与蛋白质表面的巯基含量为指标,探讨了不同温度冻藏过程(-10、-20、-30、-40℃)对虾虾肉蛋白质生物化学特性的变化,评价了不同冻藏温度对对虾品质的影响。结果表明,第一,3种蛋白质的冷冻变性指标应用于对虾时出现一定差异。Ca2+-ATPase活性比较灵敏,能较好反映-10～-40℃不同冻藏温度引起的蛋白质变性的程度。肌原纤维蛋白盐溶解度能够反映-20℃以上冻藏过程中蛋白质变性程度的差异,而-20～-40℃的差异难以区分。-10℃冻藏时,肌原纤维蛋白的盐溶解度下降明显,而-20℃冻藏时下降程度较小,-20、-30、-40℃冻藏时肌原纤维蛋白的盐溶解度没有明显区别。-10～-40℃冻藏对于巯基含量变化都不大。第二,冻藏过程中3种对虾蛋白质冷冻变性较轻度,比一般鱼类在冻藏过程中稳定,虾比大部分鱼类更耐冻。第三,冻藏温度越低Ca2+-ATPase活性下降程度越小,-10～-40℃冻藏时其差异性有比较明显区分,表明Ca2+-ATPase活性更适合于虾的质量评价。第四,-20℃冻藏对于虾类是比较经济合理的,实用冻藏温度可取-20℃...

Biochemical changes in prawn protein during frozen storage

Prawn is widely popular because of its nutrition and delicacy. The technology and scale of prawn aquaculture developed rapidly with high economic value. The prosperity of production and marketing grow continuously. Frozen storage,including frozen prawn,is widely used as a good method of preservation and processing. However,characteristics of prawn frozen storage and the impact on prawn quality had not been deeply studied yet. Three kinds of cultured prawn,dominanted in market,were chosen as study objects,an...