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鳞砗磲的人工繁育和早期发生
引用本文:张跃环,肖述,李军,马海涛,向志明,张扬,喻子牛.鳞砗磲的人工繁育和早期发生[J].水产学报,2016,40(11):1713-1723.
作者姓名:张跃环  肖述  李军  马海涛  向志明  张扬  喻子牛
作者单位:中国科学院南海海洋研究所,热带海洋生物资源与生态重点实验室,广东省应用海洋生物学重点实验室,广东广州510301;南海生物资源开发与利用协同创新中心,广东广州510275
基金项目:中国科学院STS项目(KFJ-EW-STS-123);战略先导A专项(XDA13020200);重点部署项目(KGZD-EW-609);国家贝类现代产业技术体系建设项目(CARS-48);广东省应用海洋生物学重点实验室运行经费(2014 B030301064)
摘    要:基于2015年的预备性试验,于2016年3—6月在三亚开展了鳞砗磲人工繁育技术研究。结果显示,采用五羟色胺催产剂可以有效促使鳞砗磲排放配子,精卵比例为50∶1~100∶1时受精率、孵化率较高,孵化密度控制在15~20个/m L较适宜,经过36 h孵化,获得初孵D形幼虫;优选的D形幼虫经过5 d的微充气培养,即发育至足面盘幼虫,进入附着变态期。利用净水采苗法促使幼虫完成变态,经过7~10 d,幼虫出现鳃、次生壳,并建立完善的虫黄藻系统,即完成变态、形成稚贝。利用一定浓度虫黄藻浸泡足面盘幼虫2 h以内,可以有效地提高变态率。采用微流水+微充气模式和2000~3000 lx光照(白天)进行稚贝培养,稚贝利用体内虫黄藻提供的营养就可以继续完成贝壳生长、器官发育。经过48 d和65 d的培育,分别成功获得壳长×壳高=(1174.0±146.5)μm×(1208.0±135.3)μm的稚贝约3万个,壳长×壳高=(1750.2±224.1)μm×(1816.5±226.5)μm的幼贝约0.5万个。本研究首次在国内人工繁育成功,获得了鳞砗磲苗种,为鳞砗磲人工大规模繁育、增殖放流、移植保育等工作奠定了基础。

关 键 词:鳞砗磲  人工繁育  幼虫培育  附着变态  虫黄藻  稚贝培养
收稿时间:2016/6/26 0:00:00
修稿时间:9/3/2016 12:00:00 AM

The artificial breeding and early development of the fluted giant clam (Tridacna squamosa) in South China Sea
ZHANG Yuehuan,XIAO Shu,LI Jun,MA Haitao,XIANG Zhiming,ZHANG Yang and YU Ziniu.The artificial breeding and early development of the fluted giant clam (Tridacna squamosa) in South China Sea[J].Journal of Fisheries of China,2016,40(11):1713-1723.
Authors:ZHANG Yuehuan  XIAO Shu  LI Jun  MA Haitao  XIANG Zhiming  ZHANG Yang and YU Ziniu
Institution:Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China,Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China,Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China,Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China,Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China,Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China and Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China
Abstract:
Keywords:Tridacna squamosa  artificial breeding  larval rearing  metamorphosis  zooxanthellae  spat culture
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