| 异育银鲫P450家族CYP3A136基因的克隆与表达 |
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| 引用本文： | 朱磊,胡晓,房文红,胡琳琳,李新苍,李国烈,写腊月.异育银鲫P450家族CYP3A136基因的克隆与表达[J].水产学报,2011,35(10):1450-1457. |
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| 作者姓名： | 朱磊 胡晓 房文红 胡琳琳 李新苍 李国烈 写腊月 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海200090;四川农业大学动物医学院,四川雅安625014
2. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海200090 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(2007M06) |
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| 摘 要： | 根据5种硬骨鱼已知CYP3A序列保守区设计兼并引物,以异育银鲫cDNA为模板扩增得到CYP3A基因片段,根据得到片段序列设计特异引物并利用cDNA末端快速扩增技术(RACE)获得全长cDNA。得到异育银鲫CYP3A基因全长为1 769 bp,开放阅读框为1 545个核苷酸,编码514个氨基酸。其预测蛋白质分子量为58.624 ku,理论等电点为6.30。将异育银鲫CYP3A序列理论编码氨基酸序列提交细胞色素P450命名委员会(Cytochrome P450 Nomenclature Committee)并由其命名为CYP3A136。氨基酸序列分析显示,其与稀有鮈鲫、草鱼、鲦同源性较高,并且具有高度保守的血红素结合区域FXXGXXXCXG。异育银鲫CYP3A基因的半定量RT-PCR显示,在肝和肠组织中转录水平最高,在肾和鳃中次之,其余组织较低。
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| 关 键 词： | 异育银鲫 CYP3A136 克隆 组织分布 |
| 收稿时间： | 2011/4/21 0:00:00 |
| 修稿时间： | 7/6/2011 8:25:44 AM |
Cloning,sequencing and tissue expression of the cytochrome P450 3A136 in crucian carp(Carassius auratus gibelio) |
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| ZHU Lei,HU Xiao,FANG Wen-hong,HU Lin-lin,LI Xin-cang,LI Guo-lie and XIE La-yue.Cloning,sequencing and tissue expression of the cytochrome P450 3A136 in crucian carp(Carassius auratus gibelio)[J].Journal of Fisheries of China,2011,35(10):1450-1457. |
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| Authors: | ZHU Lei HU Xiao FANG Wen-hong HU Lin-lin LI Xin-cang LI Guo-lie and XIE La-yue |
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| Institution: | East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,CAFS,Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture |
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| Abstract: | |
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| Keywords: | crucian carp(Carassius auratus gibelio) CYP3A136 cloning tissue expression |
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